Controlled releasing of SDF-1α in chitosan-heparin hydrogel for endometrium injury healing in rat model.

Autor: Wenbo Q; Department of Obstetrics and Gynecology, The Second Hospital of Hebei Medical University, 215 Heping West Road, Shijiazhuang 050000, China., Lijian X; Shandong Univ, Sch Chem & Chem Engn, Jinan 250100, China., Shuangdan Z; Department of Obstetrics and Gynecology, The Second Hospital of Hebei Medical University, 215 Heping West Road, Shijiazhuang 050000, China., Jiahua Z; Department of Obstetrics and Gynecology, The Second Hospital of Hebei Medical University, 215 Heping West Road, Shijiazhuang 050000, China., Yanpeng T; Department of Obstetrics and Gynecology, The Second Hospital of Hebei Medical University, 215 Heping West Road, Shijiazhuang 050000, China., Xuejun Q; Department of Obstetrics and Gynecology, The Second Hospital of Hebei Medical University, 215 Heping West Road, Shijiazhuang 050000, China., Xianghua H; Department of Obstetrics and Gynecology, The Second Hospital of Hebei Medical University, 215 Heping West Road, Shijiazhuang 050000, China. Electronic address: huangxh2003@163.com., Jingkun Z; Department of Obstetrics and Gynecology, The Second Hospital of Hebei Medical University, 215 Heping West Road, Shijiazhuang 050000, China.
Jazyk: angličtina
Zdroj: International journal of biological macromolecules [Int J Biol Macromol] 2020 Jan 15; Vol. 143, pp. 163-172. Date of Electronic Publication: 2019 Nov 22.
DOI: 10.1016/j.ijbiomac.2019.11.184
Abstrakt: Herein we report a facile approach for chitosan-heparin hydrogels with controlled release manner and their applications for intrauterine adhesion. The sol precursor was converted to gel at physiological temperature in 15 min. FTIR, SEM and swelling test were performed to characterize their compositions, morphologies and stability. In vitro releasing profiles was investigated in PBS solutions. Intrauterine injured rat model was established and treated with different methods. The results of H&E staining, Masson trichrome staining, western blots assay, immunohistochemical staining and immunofluorescence staining revealed that endogenous c-kit positive stem cells (HSCs) were recruited to the injury site and promoted the wound recovery. After 7 days' treatment, uterus treated with SDF-1α releasing hydrogel showed no difference with control group on endometrial thickness, glands number and fibrosis level. This work provides a possible method for intrauterine adhesion healing.
(Copyright © 2019 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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