| Autor: |
Huang JC; Department of Ophthalmology, Oregon Health & Science University (OHSU), Portland, Oregon, USA.; Department of Ophthalmology, Chang Gung Memorial Hospital, Keelung, Taiwan.; Graduate Institute of Clinical Medical Sciences, Chang Gung University, Taoyuan, Taiwan., Schleisman M; Department of Ophthalmology, Oregon Health & Science University (OHSU), Portland, Oregon, USA., Choi D; OHSU-PSU School of Public Health, Oregon Health & Science University and Graduate School of Dentistry, Kyung Hee University, Seoul, Korea., Mitchell C; Department of Ophthalmology, Oregon Health & Science University (OHSU), Portland, Oregon, USA., Watson L; Department of Ophthalmology, Oregon Health & Science University (OHSU), Portland, Oregon, USA., Asquith M; Department of Medicine, OHSU, Portland, Oregon, USA., Rosenbaum JT; Departments of Ophthalmology, Medicine, and Cell Biology, OHSU, Portland, Oregon, USA.; Department of Ophthalmology, Legacy Devers Eye Institute, Portland, Oregon, USA. |
| Abstrakt: |
Purpose: Anterior uveitis is the most common anatomic subset of uveitis. We developed a novel multi-parametric flow cytometry panel to identify immune dysregulation signatures in HLA B27-associated acute anterior uveitis (AAU) and axial spondyloarthritis (AxSpA). Methods: We used fluorescence activated cell sorting to characterize T cell cytokine expression in stimulated T cell subsets from patients with AAU (n = 4) compared to healthy controls (n = 14) or subjects with AxSpA (n = 6). Results: Positive findings among subjects with AAU included a statistically significant increase in stimulated granulocyte-macrophage colony stimulating factor (GM-CSF), IL-17, and IL-22 synthesized by CD8 cells, a trend for stimulated ILC (innate lymphoid cells)-3 cells to synthesize more IL-22 ( p = .07), and stimulated MAIT (mucosa associated innate lymphoid cells)-like cells that express the T cell receptor V alpha 7.2 to express IL-17A, IL-17F, and IL-22 in a greater percentage of cells relative to controls. IL-17F, GM- CSF, and IL-22 represent potentially novel targets in AAU. Conclusion: Our report is arguably the first to implicate IL-17F or ILC-3 and MAIT cells in the pathogenesis of AAU. Abbreviations AAU: acute anterior uveitis; AxSpA: axial spondyloarthritis; BASDAI: Bath ankylosing spondylitis disease activity index; CCR: chemokine receptor; DMSO: dimethylsulfoxide; EULAR:European League Against Rheumatism; FACS: fluorescence activated cell sorter; FBS: fetal bovine serum; FSC: orward light scatter; GM-CSF: granulocyte-macrophage colony stimulating factor; HC: healthy control; ILC: innate lymphoid cell; KIR: killer immunoglobulin receptor; MAIT: mucosal associated immune T cell; ND: not detected; NK: natural killer cell; OHSU-Oregon Health & Science University; PBMC: peripheral blood mononuclear cell; SSC: side light scatter; TCR: T cell receptor. |
