Effects of Temperature on the Ability of Metabolites from Permafrost Microorganisms to Activate the Synthesis of Systemic Cytokines by Mononuclear Cells.

Autor: Kalenova LF; Tyumen State University, Tyumen, Russia. lkalenova@mail.ru.; Federal Research Centre Tyumen Scientific Centre, Siberian Division of the Russian Academy of Sciences, Tyumen, Russia. lkalenova@mail.ru., Kolyvanova SS; Tyumen State University, Tyumen, Russia.; Federal Research Centre Tyumen Scientific Centre, Siberian Division of the Russian Academy of Sciences, Tyumen, Russia.
Jazyk: angličtina
Zdroj: Bulletin of experimental biology and medicine [Bull Exp Biol Med] 2019 Nov; Vol. 168 (1), pp. 72-75. Date of Electronic Publication: 2019 Nov 25.
DOI: 10.1007/s10517-019-04650-6
Abstrakt: The effects of metabolites of Bacillus sp. strain M3 and Bacillus megaterium strain 8/75-1 isolated from permafrost formations, as well as a medicinal Bacillus cereus strain IP5832 on the secretion of TNFα, IL-1β, and IL-10 by mononuclear cells of human peripheral blood depending on the temperature of bacteria culturing (-16oC, -5oC, and 42oC) were studied. Metabolites of 8/75-1 and M3 strains cultured at 42oC produced more potent stimulating effect on TNFα and IL-10 synthesis than metabolites from these bacteria cultured at -16oC and -5oC (p<0.01). Temperature did not affect the ability of metabolites to modulate IL-1β synthesis in all bacterial strains and the ability of IP5832 strain metabolites to modulate TNFα production. IL-10 secretion was minimum under the effect of metabolites of IP5832 strain cultured at 42oC. The TNFα/IL-10 and IL-1β/IL-10 ratios indicated that metabolites of permafrost bacteria cultured at 42oC can shift the balance towards the synthesis of proinflammatory cytokines.
Databáze: MEDLINE
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