Exploring the genetic potential of a fosmid metagenomic library from an oil-impacted mangrove sediment for metabolism of aromatic compounds.

Autor: Sousa STP; Research Center for Chemistry, Biology and Agriculture (CPQBA), Institute of Biology, University of Campinas (UNICAMP), Campinas, São Paulo, Brazil. Electronic address: sousa.tarciso@gmail.com., Cabral L; Research Center for Chemistry, Biology and Agriculture (CPQBA), Institute of Biology, University of Campinas (UNICAMP), Campinas, São Paulo, Brazil. Electronic address: luc.g.cabral@gmail.com., Lacerda-Júnior GV; Research Center for Chemistry, Biology and Agriculture (CPQBA), Institute of Biology, University of Campinas (UNICAMP), Campinas, São Paulo, Brazil. Electronic address: gilenolacerdajr@gmail.com., Noronha MF; Research Center for Chemistry, Biology and Agriculture (CPQBA), Institute of Biology, University of Campinas (UNICAMP), Campinas, São Paulo, Brazil. Electronic address: melfontes@gmail.com., Ottoni JR; Research Center for Chemistry, Biology and Agriculture (CPQBA), Institute of Biology, University of Campinas (UNICAMP), Campinas, São Paulo, Brazil. Electronic address: ottoni.julia@gmail.com., Sartoratto A; Research Center for Chemistry, Biology and Agriculture (CPQBA), Institute of Biology, University of Campinas (UNICAMP), Campinas, São Paulo, Brazil. Electronic address: adilson@cpqba.unicamp.br., Oliveira VM; Research Center for Chemistry, Biology and Agriculture (CPQBA), Institute of Biology, University of Campinas (UNICAMP), Campinas, São Paulo, Brazil. Electronic address: vmaia@cpqba.unicamp.br.
Jazyk: angličtina
Zdroj: Ecotoxicology and environmental safety [Ecotoxicol Environ Saf] 2020 Feb; Vol. 189, pp. 109974. Date of Electronic Publication: 2019 Nov 21.
DOI: 10.1016/j.ecoenv.2019.109974
Abstrakt: Aromatic hydrocarbons (AH) are widely distributed in nature, and many of them have been reported as relevant environmental pollutants and valuable carbon sources for different microorganisms. In this work, high-throughput sequencing of a metagenomic fosmid library was carried out to evaluate the functional and taxonomic diversity of genes involved in aromatic compounds degradation in oil-impacted mangrove sediments. In addition, activity-based approach and gas chromatography were used to assess the degradation potential of fosmid clones. Results indicated that AH degradation genes, such as monooxygenases and dioxygenases, were grouped into the following categories: anaerobic degradation of aromatic compounds (20.34%), metabolism of central aromatic intermediates (35.40%) and peripheral pathways for catabolism of aromatic compounds (22.56%). Taxonomic affiliation of genes related to aromatic compounds metabolism revealed the prevalence of the classes Alphaproteobacteria, Actinobacteria, Betaproteobacteria, Gammaproteobacteria and Deltaproteobacteria. Aromatic hydrocarbons (phenol, naphthalene, phenanthrene, pyrene and benzopyrene) were used as the only carbon source to screen clones with degradation potential. Of the 2500 clones tested, 48 showed some respiratory activity in at least one of the five carbon sources used. The hydrocarbon degradation ability of the top ten fosmid clones was confirmed by GC-MS. Further, annotation of assembled metagenomic fragments revealed ORFs corresponding to proteins and functional domains directly or indirectly involved in the aromatic compound metabolism, such as catechol 2,3-dioxygenase and ferredoxin oxidoreductase. Finally, these data suggest that the indigenous mangrove sediment microbiota developed essential mechanisms towards ecosystem remediation of petroleum hydrocarbon impact.
(Copyright © 2019 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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