Molecular characterization and RSV Co-infection of Nicotiana benthamiana with three distinct begomoviruses.

Autor: Arif M; Fujian Province Key Laboratory of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China; Department of Plant Protection, Faculty of Agricultural Sciences, Ghazi University, Dera Ghazi Khan, Punjab 32200, Pakistan; Department of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou, Fujian, China. Electronic address: arif_1821uaf@yahoo.com., Atta S; Department of Plant Protection, Faculty of Agricultural Sciences, Ghazi University, Dera Ghazi Khan, Punjab 32200, Pakistan., Bashir MA; Department of Plant Protection, Faculty of Agricultural Sciences, Ghazi University, Dera Ghazi Khan, Punjab 32200, Pakistan., Hussain A; Department of Plant Breeding and Genetics, Faculty of Agricultural Sciences, Ghazi University, Dera Ghazi Khan, Punjab 32200, Pakistan., Khan MI; Department of Plant Breeding and Genetics, Faculty of Agricultural Sciences, Ghazi University, Dera Ghazi Khan, Punjab 32200, Pakistan., Farooq S; Department of Plant Protection, Faculty of Agriculture, Harran University, Sanliurfa 63200, Turkey., Hannan A; Department of Botany, Ghazi University, Dera Ghazi Khan, Punjab 32200, Pakistan., Islam SU; Department of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou, Fujian, China., Umar UUD; Department of Plant Pathology, Bahauddin Zakariya University, Multan, Punjab 60800, Pakistan., Khan M; Department of Plant Protection, Faculty of Agricultural Sciences, Ghazi University, Dera Ghazi Khan, Punjab 32200, Pakistan., Lin W; Fujian Province Key Laboratory of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China; Department of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou, Fujian, China., Hashem M; King Khalid University, College of Science, Department of Biology, Abha 61413, Saudi Arabia; Assiut University, Faculty of Science, Botany and Microbiology Department, Assiut, Egypt., Alamri S; King Khalid University, College of Science, Department of Biology, Abha 61413, Saudi Arabia; Research Center for Advanced Materials Science (RCAMS), King Khalid University, Abha 61413, Saudi Arabia., Wu Z; Fujian Province Key Laboratory of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China; Department of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou, Fujian, China. Electronic address: wuzujian@126.com.
Jazyk: angličtina
Zdroj: Methods (San Diego, Calif.) [Methods] 2020 Nov 01; Vol. 183, pp. 43-49. Date of Electronic Publication: 2019 Nov 20.
DOI: 10.1016/j.ymeth.2019.11.010
Abstrakt: Geminiviruses constitute a family of plant viruses with characteristic twinned quasi-icosahedral virions and a small circular DNA genome. Geminiviruses, especially begomoviruses, cause substantial economic losses in tropical and subtropical regions globally. Geminiviruses use the host's transcriptional mechanisms to synthesize their mRNAs. They are considered as an attractive model to understand the transcription mechanism of their host plants. Experiments were conducted to identify transcriptional start sites (TSSs) of the three begomoviruses, i.e., Cotton leaf curl Multan virus (CLCuMuV), Corchorus yellow vein virus (CoYVV), and Ramie mosaic virus (RamV). We first rub-inoculated Rice stripe tenuivirus (RSV), a segmented negative-sense RNA virus that uses cap-snatching to produce capped viral mRNAs, into N. benthamiana. After the inoculation, RSV-infected N. benthamiana were super-infected by CoYVV, CLCuMuV, or RamV, respectively. The capped-RNA leaders snatched by RSV were obtained by determining the 5'-ends of RSV mRNA with high throughput sequencing. Afterwards, snatched capped-RNA leaders of RSV were mapped onto the genome of each begomovirus and those matching the begomoviral genome were considered to come from the 5' ends of assumed begomoviral mRNAs. In this way, TSSs of begomoviruses were obtained. After mapping these TSSs onto the genome of the respective begomovirus, it was found very commonly that a begomovirus can use many different TSSs to transcribe the same gene, producing many different mRNA isoforms containing the corresponding open reading frames (ORFs).
(Copyright © 2019 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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