Lipoproteolytic capacity and potential of Pseudomonas spp. isolated from cold raw milk.

Autor: de Moura Aguiar B; Mestrado em Ciência e Tecnologia de Leite e Derivados., de Longhi R; Mestrado em Ciência e Tecnologia de Leite e Derivados., Poli-Frederico RC; Mestrado e Doutorado em Ciência da Reabilitação, Universidade Pitágoras Unopar - UNOPAR, Londrina, Brazil., Fagnani R; Mestrado em Ciência e Tecnologia de Leite e Derivados., de Santana EHW; Mestrado em Ciência e Tecnologia de Leite e Derivados.
Jazyk: angličtina
Zdroj: The Journal of dairy research [J Dairy Res] 2019 Nov; Vol. 86 (4), pp. 467-469. Date of Electronic Publication: 2019 Nov 22.
DOI: 10.1017/S0022029919000645
Abstrakt: The objective of the work described in this research communication was to determine the lipoproteolytic capacity and potential of Pseudomonas spp. from the microbiota of refrigerated raw milk. The influence of temperature and bacterial population on these activities was also evaluated. Pseudomonas spp. (PS) counts (30 °C/48 h) were confirmed by PCR. Proteolytic (10% milk agar) and lipolytic capacities (PLC) (tributyrin agar) were evaluated (21 °C/72 h). Proteolytic (PP) and lipolytic potential (LP) were assessed by measuring the diameter of the halos and were categorized as low or high. A total of 91.3% PS possessed PLC. The PP of 64.16% isolates was high and was frequently observed in PS from milk samples with higher counts and lower temperatures. The LP of 70.52% isolates was low, and higher LP was associated with low microbiological counts and temperatures. Genetic studies evaluating Pseudomonas spp. strains in the milking environment and investigating the origin of these isolates could be useful to improve the quality and shelf life of dairy products.
Databáze: MEDLINE