A Mini-Atlas of Gene Expression for the Domestic Goat ( Capra hircus ).

Autor:	Muriuki C; The Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, Edinburgh, United Kingdom.; Centre for Tropical Livestock Genetics and Health (CTLGH), Edinburgh, United Kingdom., Bush SJ; The Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, Edinburgh, United Kingdom.; Nuffield Department of Clinical Medicine, John Radcliffe Hospital, University of Oxford, Oxford, United Kingdom., Salavati M; The Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, Edinburgh, United Kingdom.; Centre for Tropical Livestock Genetics and Health (CTLGH), Edinburgh, United Kingdom., McCulloch MEB; The Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, Edinburgh, United Kingdom., Lisowski ZM; The Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, Edinburgh, United Kingdom., Agaba M; Biosciences Eastern and Central Africa - International Livestock Research Institute (BecA - ILRI) Hub, Nairobi, Kenya., Djikeng A; Centre for Tropical Livestock Genetics and Health (CTLGH), Edinburgh, United Kingdom., Hume DA; Mater Research Institute-University of Queensland, Woolloongabba, QLD, Australia., Clark EL; The Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, Edinburgh, United Kingdom.; Centre for Tropical Livestock Genetics and Health (CTLGH), Edinburgh, United Kingdom.
Jazyk:	angličtina
Zdroj:	Frontiers in genetics [Front Genet] 2019 Nov 04; Vol. 10, pp. 1080. Date of Electronic Publication: 2019 Nov 04 (Print Publication: 2019).
DOI:	10.3389/fgene.2019.01080
Abstrakt:	Goats ( Capra hircus ) are an economically important livestock species providing meat and milk across the globe. They are of particular importance in tropical agri-systems contributing to sustainable agriculture, alleviation of poverty, social cohesion, and utilisation of marginal grazing. There are excellent genetic and genomic resources available for goats, including a highly contiguous reference genome (ARS1). However, gene expression information is limited in comparison to other ruminants. To support functional annotation of the genome and comparative transcriptomics, we created a mini-atlas of gene expression for the domestic goat. RNA-Seq analysis of 17 transcriptionally rich tissues and 3 cell-types detected the majority (90%) of predicted protein-coding transcripts and assigned informative gene names to more than 1000 previously unannotated protein-coding genes in the current reference genome for goat (ARS1). Using network-based cluster analysis, we grouped genes according to their expression patterns and assigned those groups of coexpressed genes to specific cell populations or pathways. We describe clusters of genes expressed in the gastro-intestinal tract and provide the expression profiles across tissues of a subset of genes associated with functional traits. Comparative analysis of the goat atlas with the larger sheep gene expression atlas dataset revealed transcriptional similarities between macrophage associated signatures in the sheep and goats sampled in this study. The goat transcriptomic resource complements the large gene expression dataset we have generated for sheep and contributes to the available genomic resources for interpretation of the relationship between genotype and phenotype in small ruminants. (Copyright © 2019 Muriuki, Bush, Salavati, McCulloch, Lisowski, Agaba, Djikeng, Hume and Clark.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu