Autophagic Survival Precedes Programmed Cell Death in Wheat Seedlings Exposed to Drought Stress.

Autor: Li YB; Crop Research Institute, Shandong Academy of Agricultural Sciences, Jinan 250100, China., Cui DZ; Crop Research Institute, Shandong Academy of Agricultural Sciences, Jinan 250100, China., Sui XX; Crop Research Institute, Shandong Academy of Agricultural Sciences, Jinan 250100, China., Huang C; Crop Research Institute, Shandong Academy of Agricultural Sciences, Jinan 250100, China., Huang CY; Crop Research Institute, Shandong Academy of Agricultural Sciences, Jinan 250100, China., Fan QQ; Crop Research Institute, Shandong Academy of Agricultural Sciences, Jinan 250100, China., Chu XS; Crop Research Institute, Shandong Academy of Agricultural Sciences, Jinan 250100, China.; School of life science, Shandong Normal University, Jinan 250100, China.
Jazyk: angličtina
Zdroj: International journal of molecular sciences [Int J Mol Sci] 2019 Nov 16; Vol. 20 (22). Date of Electronic Publication: 2019 Nov 16.
DOI: 10.3390/ijms20225777
Abstrakt: Although studies have shown the concomitant occurrence of autophagic and programmed cell death (PCD) in plants, the relationship between autophagy and PCD and the factors determining this relationship remain unclear. In this study, seedlings of the wheat cultivar Jimai 22 were used to examine the occurrence of autophagy and PCD during polyethylene glycol (PEG)-8000-induced drought stress. Autophagy and PCD occurred sequentially, with autophagy at a relatively early stage and PCD at a much later stage. These findings suggest that the duration of drought stress determines the occurrence of PCD following autophagy. Furthermore, the addition of 3-methyladenine (3-MA, an autophagy inhibitor) and the knockdown of autophagy-related gene 6 (ATG6) accelerated PEG-8000-induced PCD, respectively, suggesting that inhibition of autophagy also results in PCD under drought stress. Overall, these findings confirm that wheat seedlings undergo autophagic survival under mild drought stress, with subsequent PCD only under severe drought.
Competing Interests: The authors declare that they have no conflicts of interest with the contents of this article.
Databáze: MEDLINE
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