Edwardsiella ictaluri evpP is required for colonisation of channel catfish ovary cells and necrosis in anterior kidney macrophages.

Autor: Kalindamar S; Department of Molecular Biology and Genetics, Faculty of Arts and Sciences, Ordu University, Ordu, Turkey., Kordon AO; Department of Basic Sciences, College of Veterinary Medicine, Mississippi State University, Mississippi State, Mississippi., Abdelhamed H; Department of Basic Sciences, College of Veterinary Medicine, Mississippi State University, Mississippi State, Mississippi., Tan W; Department of Basic Sciences, College of Veterinary Medicine, Mississippi State University, Mississippi State, Mississippi., Pinchuk LM; Department of Basic Sciences, College of Veterinary Medicine, Mississippi State University, Mississippi State, Mississippi., Karsi A; Department of Basic Sciences, College of Veterinary Medicine, Mississippi State University, Mississippi State, Mississippi.
Jazyk: angličtina
Zdroj: Cellular microbiology [Cell Microbiol] 2020 Mar; Vol. 22 (3), pp. e13135. Date of Electronic Publication: 2019 Dec 20.
DOI: 10.1111/cmi.13135
Abstrakt: Edwardsiella ictaluri is a Gram-negative facultative anaerobe that can survive inside channel catfish phagocytes. E. ictaluri can orchestrate Type VI Secretion System (T6SS) for survival in catfish macrophages. evpP encodes one of the T6SS translocated effector proteins. However, the role of evpP in E. ictaluri is still unexplored. In this work, we constructed an E. ictaluri evpP mutant (EiΔevpP) and assessed its survival under complement and oxidative stress. Persistence of EiΔevpP in catfish as well as attachment and invasion in catfish macrophage and ovary cells were determined. Further, virulence of EiΔevpP in catfish and apoptosis it caused in macrophages were explored. EiΔevpP behaved same as wild type (EiWT) under complement and oxidative stress in complex media, whereas oxidative stress affected mutant's survival significantly in minimal media (p < .05). Persistence of EiΔevpP in live catfish and uptake and survival inside peritoneal macrophages were similar. The attachment and invasion capabilities of EiΔevpP in catfish ovary cells were significantly less than that of EiWT (p < .05). Although EiΔevpP showed reduced attenuation in catfish, causing decreased catfish mortality compared with EiWT (44.73% vs. 67.53%), this difference was not significant. The apoptosis assay using anterior kidney macrophages indicated that the number of live macrophages exposed to EiΔevpP was significantly higher compared with EiWT exposed macrophages at 24-hr post-treatment (p < .05). However, there were no significant differences in the early and late apoptosis. Remarkably, necrosis in EiΔevpP exposed macrophages was significantly less than that of EiWT exposed macrophages at 24 hr (p < .05). Our results demonstrated that evpP is required for colonisation of catfish ovary cells and increased apoptosis and necrosis in anterior kidney macrophages.
(© 2019 John Wiley & Sons Ltd.)
Databáze: MEDLINE
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