Presence of Archaea in dental caries biofilms.

Autor: Dame-Teixeira N; Department of Dentistry, Faculty of Heath Sciences, University of Brasília, Brasília, DF, Brazil. Electronic address: nailedame@hotmail.com., de Cena JA; Department of Dentistry, Faculty of Heath Sciences, University of Brasília, Brasília, DF, Brazil., Côrtes DA; Department of Cell Biology, Institute of Biological Sciences, University of Brasília, Brasília, DF, Brazil., Belmok A; Department of Cell Biology, Institute of Biological Sciences, University of Brasília, Brasília, DF, Brazil., Dos Anjos Borges LG; Institute of Petroleum and Natural Resources, Pontifical Catholic University of Rio Grande do Sul, Porto Alegre, RS, Brazil., Marconatto L; Institute of Petroleum and Natural Resources, Pontifical Catholic University of Rio Grande do Sul, Porto Alegre, RS, Brazil., Giongo A; Institute of Petroleum and Natural Resources, Pontifical Catholic University of Rio Grande do Sul, Porto Alegre, RS, Brazil., Kyaw CM; Department of Cell Biology, Institute of Biological Sciences, University of Brasília, Brasília, DF, Brazil.
Jazyk: angličtina
Zdroj: Archives of oral biology [Arch Oral Biol] 2020 Feb; Vol. 110, pp. 104606. Date of Electronic Publication: 2019 Nov 09.
DOI: 10.1016/j.archoralbio.2019.104606
Abstrakt: Objective: Although the prevalence and functions associated with members of Bacteria are well known in dental caries, the role of Archaea in cariogenic biofilms has not been studied yet.
Design: To detect the presence of Archaea in dental caries, a triplicate of carious dentine samples and duplicate of supragingival biofilms were collected, total microbial DNA was extracted and the composition of the microbiota was investigated. Total DNA was submitted to 16S rRNA gene amplification using universal prokaryotic primers; amplicons were sequenced by high-throughput DNA sequencing. As a second strategy to detect Archaea, a representative sample of caries was chosen and other PCR reactions were performed using specific primers targeting the archaeal 16S rRNA gene; amplicons were cloned and sequenced. Annotation of sequences was performed using SILVA database and the relative abundance of genus level OTUs was calculated.
Results: The high-throughput sequencing method detected archaeal sequences in all samples (identified as group I.1c of the phylum Thaumarchaeota), although in a very low abundance (≤0.03 % of the total sequences). For the second strategy, 14 archaeal clones were detected, with an OTU affiliated to Methanocella clade, and another one affiliated to group I.1b of the phylum Thaumarchaeota.
Conclusions: Archaeal sequences were detected in dental caries and biofilms from surfaces without caries lesions. DNA sequences of Thaumarchaeota were also identified, showing that overall archaeal diversity in the human oral cavity could be currently underestimated and not restricted to methanogens.
(Copyright © 2019 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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