Pluronic F-127: An Efficient Delivery Vehicle for 3-(1'-hexyloxy)ethyl-3-devinylpyropheophorbide-a (HPPH or Photochlor).

Autor: Cacaccio J; Cell Stress Biology, PDT Center, Roswell Park Cancer Institute, Buffalo, NY., Durrani F; Cell Stress Biology, PDT Center, Roswell Park Cancer Institute, Buffalo, NY., Cheruku RR; Cell Stress Biology, PDT Center, Roswell Park Cancer Institute, Buffalo, NY., Borah B; Photolitec, LLC, Buffalo, NY., Ethirajan M; Cell Stress Biology, PDT Center, Roswell Park Cancer Institute, Buffalo, NY., Tabaczynski W; Photolitec, LLC, Buffalo, NY., Pera P; Cell Stress Biology, PDT Center, Roswell Park Cancer Institute, Buffalo, NY., Missert JR; Cell Stress Biology, PDT Center, Roswell Park Cancer Institute, Buffalo, NY., Pandey RK; Cell Stress Biology, PDT Center, Roswell Park Cancer Institute, Buffalo, NY.
Jazyk: angličtina
Zdroj: Photochemistry and photobiology [Photochem Photobiol] 2020 May; Vol. 96 (3), pp. 625-635. Date of Electronic Publication: 2019 Dec 26.
DOI: 10.1111/php.13183
Abstrakt: To determine the impact of delivery vehicles in photosensitizing efficacy of HPPH, a hydrophobic photosensitizer was dissolved in various formulations: 1% Tween 80/5% dextrose, Pluronic P-123 and Pluronic F-127 in 0.5%, 1% and 2% phosphate buffer solutions (PBS). HPPH was also conjugated to Pluronic F-127, and the resulting conjugate (PL-20) was formulated in PBS. Among the different delivery vehicles, only Pluronic P-123 displayed significant vehicle cytotoxicity, whereas Pluronic F127 was nontoxic. Compared to PL-20, HPPH formulated in Tween80 and Pluronic F-127 showed higher cell-uptake, but lower long-term retention in Colon26 cell compared to PL-20. The higher retention of PL-20 was similarly observed during in vivo uptake with BALB/c mice baring Ct26 tumors. In contrast to the in vitro uptake experiments, PL-20 showed slightly higher uptake compared to HPPH formulated in Tween or Pluronic-F127. A significant difference in pharmacokinetic profile was also observed between the HPPH-Pluronic formulation and PL-20. Under similar in vivo treatment parameters (drug dose 0.47 µmol kg -1 , light dose: 135 J cm -2 at 24 h post-injection of PS), HPPH formulated either in Tween or Pluronic F-127 formulation showed similar in vivo PDT efficacy (20-30% tumor cure on day 60), whereas PL-20 showed 40% tumor cure (day 60).
(© 2019 American Society for Photobiology.)
Databáze: MEDLINE
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