| Autor: |
Teng JLL; Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, PR China.; State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Hong Kong, PR China.; Carol Yu Centre for Infection, The University of Hong Kong, Hong Kong, PR China., Fong JYH; Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, PR China., Fok KMN; Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, PR China., Lee HH; Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, PR China., Chiu TH; Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, PR China., Tang Y; Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, PR China., Ngan AHY; Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, PR China., Wong SSY; Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, PR China.; State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Hong Kong, PR China.; Carol Yu Centre for Infection, The University of Hong Kong, Hong Kong, PR China., Que TL; Department of Pathology, Tuen Mun Hospital, Hong Kong, PR China., Lau SKP; Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, PR China.; State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Hong Kong, PR China.; Carol Yu Centre for Infection, The University of Hong Kong, Hong Kong, PR China.; Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The University of Hong Kong, Hong Kong, PR China., Woo PCY; Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, PR China.; State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Hong Kong, PR China.; Carol Yu Centre for Infection, The University of Hong Kong, Hong Kong, PR China.; Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The University of Hong Kong, Hong Kong, PR China. |
| Abstrakt: |
Three bacterial strains, HKU70 T , HKU71 T and HKU72 T , were isolated from the conjunctival swab, blood and sputum samples of three patients with conjunctivitis, bacteraemia and respiratory infection, respectively, in Hong Kong. The three strains were aerobic, Gram-stain positive, catalase-positive, non-sporulating and non-motile bacilli and exhibited unique biochemical profiles distinguishable from currently recognized Tsukamurella species. 16S rRNA, secA , rpoB and groEL gene sequence analyses revealed that the three strains shared 99.6-99.9, 94.5-96.8, 95.7-97.8 and 97.7-98.9 % nucleotide identities with their corresponding closest Tsukamurella species respectively. DNA-DNA hybridization confirmed that they were distinct from other known species of the genus Tsukamurella (26.2±2.4 to 36.8±1.2 % DNA-DNA relatedness), in line with results of in silico genome-to-genome comparison (32.2-40.9 % Genome-to-Genome Distance Calculator and 86.3-88.9 % average nucleotide identity values]. Fatty acids, mycolic acids, cell-wall sugars and peptidoglycan analyses showed that they were typical of members of Tsukamurella . The G+C content determined based on the genome sequence of strains HKU70 T , HKU71 T and HKU72 T were 69.9, 70.2 and 70.5 mol%, respectively. Taken together, our results supported the proposition and description of three new species, i.e. Tsukamurella sputi HKU70 T (=JCM 33387 T =DSM 109106 T ) sp. nov., Tsukamurella asaccharolytica HKU71 T (=JCM 33388 T =DSM 109107 T ) sp. nov. and Tsukamurella conjunctivitidis HKU72 T (=JCM 33389 T =DSM 109108 T ) sp. nov. |
