Effect of therapeutic ultrasound on the quadriceps muscle injury in rats - evaluation of oxidative stress and inflammatory process.

Autor:	Rosa CGS; Universidade Luterana do Brasil, ULBRA, Graduate Program in Cellular and Molecular Biology Applied to Health Brasil.; Universidade Luterana do Brasil, Laboratory of Oxidative Stress and Antioxidants Brasil., Schemitt EG; Universidade Federal do Rio Grande do Sul, UFRGS, Graduate Program in Medicine, Medical Sciences Brasil.; Universidade Luterana do Brasil, Laboratory of Oxidative Stress and Antioxidants Brasil.; Hospital de Clínicas de Porto Alegre, HCPA, Laboratory of Experimental Hepatology and Gastroenterology Brasil., Hartmann RM; Universidade Federal do Rio Grande do Sul, UFRGS, Graduate Program in Medicine, Medical Sciences Brasil.; Universidade Luterana do Brasil, Laboratory of Oxidative Stress and Antioxidants Brasil.; Hospital de Clínicas de Porto Alegre, HCPA, Laboratory of Experimental Hepatology and Gastroenterology Brasil., Colares JR; Universidade Federal do Rio Grande do Sul, UFRGS, Graduate Program in Medicine, Medical Sciences Brasil.; Universidade Luterana do Brasil, Laboratory of Oxidative Stress and Antioxidants Brasil.; Hospital de Clínicas de Porto Alegre, HCPA, Laboratory of Experimental Hepatology and Gastroenterology Brasil., de Sousa JT; Universidade Luterana do Brasil, Laboratory of Oxidative Stress and Antioxidants Brasil., Bona S; Universidade Federal do Rio Grande do Sul, UFRGS, Graduate Program in Medicine, Medical Sciences Brasil.; Universidade Luterana do Brasil, Laboratory of Oxidative Stress and Antioxidants Brasil.; Hospital de Clínicas de Porto Alegre, HCPA, Laboratory of Experimental Hepatology and Gastroenterology Brasil., Moreira AJ; Universidade Luterana do Brasil, Laboratory of Oxidative Stress and Antioxidants Brasil.; Hospital de Clínicas de Porto Alegre, HCPA, Laboratory of Experimental Hepatology and Gastroenterology Brasil.; Centro Universitário Metodista, IPA, Department of Physiotherapy Brasil., Ostjen CA; Universidade Luterana do Brasil, Laboratory of Oxidative Stress and Antioxidants Brasil., Picada JN; Universidade Luterana do Brasil, ULBRA, Graduate Program in Cellular and Molecular Biology Applied to Health Brasil., Campani DP; Centro Universitário Metodista, IPA, Department of Physiotherapy Brasil., Dias AS; Hospital de Clínicas de Porto Alegre, HCPA, Laboratory of Experimental Hepatology and Gastroenterology Brasil.; Hospital de Clínicas de Porto Alegre, HCPA, Physiotherapy Service Brasil., Marroni NAP; Universidade Federal do Rio Grande do Sul, UFRGS, Graduate Program in Medicine, Medical Sciences Brasil.; Hospital de Clínicas de Porto Alegre, HCPA, Laboratory of Experimental Hepatology and Gastroenterology Brasil.
Jazyk:	angličtina
Zdroj:	American journal of translational research [Am J Transl Res] 2019 Oct 15; Vol. 11 (10), pp. 6660-6671. Date of Electronic Publication: 2019 Oct 15 (Print Publication: 2019).
Abstrakt:	Muscle injuries are frequent, both in sports and work, and may be caused by stretching, distension, repetitive effort or bruising. Such lesions can lead to the generation of free radicals, triggering oxidative stress and the release of some inflammatory mediators. Therapeutic ultrasound (UST) is one of the most used electrotherapy resources in the physiotherapist's clinical practice. Our aim was to evaluate the use of therapeutic ultrasound on oxidative stress and inflammatory process in an experimental model of single quadriceps muscle injury in Wistar rats. We used a total of 28 male rats, weighing between 250-300 grams, randomly divided into four groups. In the right quadriceps, a simple impact of contusion was induced by means of a press. The animals were submitted to a daily UST treatment for a total of seven consecutive applications for three minutes each, that started 24 hours after the trauma induction. The results in the Trauma + Therapeutic ultrasound group at TBARS levels and in the enzymatic activity of SOD and GPx presented a significant difference. In the histological analysis of the Trauma + Therapeutic ultrasound group presented a reorganization of the fiber's structure and a reduction of the presence of inflammatory infiltrate. In the results of the immunohistochemistry of iNOS, TNF-α and NF-κB in muscle tissue, we observed that the group treated with ultrasound showed a reduction in the expression of the proteins. The use of UST was effective in protecting muscle tissue from oxidative stress, inflammatory process and in the rearrangement of muscle fibers. Competing Interests: None. (AJTR Copyright © 2019.)
Databáze:	MEDLINE
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