| Autor: |
Devanadera MKP; The Graduate School, University of Santo Tomas.; Laboratory of Pure and Applied Microbiology, Research Center for the Natural and Applied Sciences, Thomas Aquinas Research Complex, University of Santo Tomas.; Department of Biochemistry, Faculty of Pharmacy, University of Santo Tomas.; Mammalian Tissue Culture Laboratory, Research Center for the Natural and Applied Sciences, Thomas Aquinas Research Complex, University of Santo Tomas., Bennett RM; Department of Biological Sciences, College of Science, University of Santo Tomas., Watanabe K; Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University., Santiago MR; The Graduate School, University of Santo Tomas.; Department of Biochemistry, Faculty of Pharmacy, University of Santo Tomas.; Mammalian Tissue Culture Laboratory, Research Center for the Natural and Applied Sciences, Thomas Aquinas Research Complex, University of Santo Tomas., Ramos MC; The Graduate School, University of Santo Tomas.; Department of Chemistry, College of Science, University of Santo Tomas., Aki T; Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University., Dedeles GR; The Graduate School, University of Santo Tomas.; Laboratory of Pure and Applied Microbiology, Research Center for the Natural and Applied Sciences, Thomas Aquinas Research Complex, University of Santo Tomas.; Department of Biological Sciences, College of Science, University of Santo Tomas. |
| Abstrakt: |
Marine oomycetes are ubiquitous, fungus-like eukaryotes known to produce fatty acids with potential anticancer activity. The long chain omega-3 and omega-6 fatty acids are currently popular and considered as safe when used as nutraceuticals in cancer treatment. In this study, crude fatty acids from three marine oomycetes, Halophytophthora spp. (T12GP1 and T12YBP2) and Salispina hoi (USTCMS 1611), were explored for their cytotoxic and apoptotic potentials against human breast adenocarcinoma cells (MCF7) and normal human dermal fibroblasts (HDFn). Extracts from mycelia mats consisted of diverse saturated, monounsaturated, and polyunsaturated fatty acids such as linoleic, α-linolenic, γ-linolenic, eicosatrienoic and eicosapentaenoic acids. The crude fatty acids from all three oomycetes in in vitro assays for cytotoxicity showed no toxicity (30% toxicity values) on HDFn cells. On MCF7 cells, however, IC 50 values of 23.44, 15.63, and 26.15 µg/mL were obtained with extracts from Halophytophthora T12GP1 and T12YBP2 and S. hoi, respectively. Treated MCF7 cells exhibited deformed cell membrane in MTT assay and also aggregation of DNA and disruption of nuclear membrane aggregation in nuclear staining; further, green signals indicative of apoptosis was recorded in caspase 3/7 assay. |
