Inhibition monitoring in veterinary molecular testing.

Autor:	Yan L; Mississippi Veterinary Research and Diagnostic Laboratory, Mississippi State University, Pearl, MS (Yan).; California Animal Health and Food Safety Laboratory, University of California-Davis, Davis, CA (Toohey-Kurth, Crossley).; Kansas State Veterinary Diagnostic Laboratory, Kansas State University, Manhattan, KS (Bai).; Department of Population Medicine and Diagnostic Sciences, Cornell University, Ithaca, NY (Glaser, Tallmadge, Goodman)., Toohey-Kurth KL; Mississippi Veterinary Research and Diagnostic Laboratory, Mississippi State University, Pearl, MS (Yan).; California Animal Health and Food Safety Laboratory, University of California-Davis, Davis, CA (Toohey-Kurth, Crossley).; Kansas State Veterinary Diagnostic Laboratory, Kansas State University, Manhattan, KS (Bai).; Department of Population Medicine and Diagnostic Sciences, Cornell University, Ithaca, NY (Glaser, Tallmadge, Goodman)., Crossley BM; Mississippi Veterinary Research and Diagnostic Laboratory, Mississippi State University, Pearl, MS (Yan).; California Animal Health and Food Safety Laboratory, University of California-Davis, Davis, CA (Toohey-Kurth, Crossley).; Kansas State Veterinary Diagnostic Laboratory, Kansas State University, Manhattan, KS (Bai).; Department of Population Medicine and Diagnostic Sciences, Cornell University, Ithaca, NY (Glaser, Tallmadge, Goodman)., Bai J; Mississippi Veterinary Research and Diagnostic Laboratory, Mississippi State University, Pearl, MS (Yan).; California Animal Health and Food Safety Laboratory, University of California-Davis, Davis, CA (Toohey-Kurth, Crossley).; Kansas State Veterinary Diagnostic Laboratory, Kansas State University, Manhattan, KS (Bai).; Department of Population Medicine and Diagnostic Sciences, Cornell University, Ithaca, NY (Glaser, Tallmadge, Goodman)., Glaser AL; Mississippi Veterinary Research and Diagnostic Laboratory, Mississippi State University, Pearl, MS (Yan).; California Animal Health and Food Safety Laboratory, University of California-Davis, Davis, CA (Toohey-Kurth, Crossley).; Kansas State Veterinary Diagnostic Laboratory, Kansas State University, Manhattan, KS (Bai).; Department of Population Medicine and Diagnostic Sciences, Cornell University, Ithaca, NY (Glaser, Tallmadge, Goodman)., Tallmadge RL; Mississippi Veterinary Research and Diagnostic Laboratory, Mississippi State University, Pearl, MS (Yan).; California Animal Health and Food Safety Laboratory, University of California-Davis, Davis, CA (Toohey-Kurth, Crossley).; Kansas State Veterinary Diagnostic Laboratory, Kansas State University, Manhattan, KS (Bai).; Department of Population Medicine and Diagnostic Sciences, Cornell University, Ithaca, NY (Glaser, Tallmadge, Goodman)., Goodman LB; Mississippi Veterinary Research and Diagnostic Laboratory, Mississippi State University, Pearl, MS (Yan).; California Animal Health and Food Safety Laboratory, University of California-Davis, Davis, CA (Toohey-Kurth, Crossley).; Kansas State Veterinary Diagnostic Laboratory, Kansas State University, Manhattan, KS (Bai).; Department of Population Medicine and Diagnostic Sciences, Cornell University, Ithaca, NY (Glaser, Tallmadge, Goodman).
Jazyk:	angličtina
Zdroj:	Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc [J Vet Diagn Invest] 2020 Nov; Vol. 32 (6), pp. 758-766. Date of Electronic Publication: 2019 Nov 18.
DOI:	10.1177/1040638719889315
Abstrakt:	Many of the sample matrices typically used for veterinary molecular testing contain inhibitory factors that can potentially reduce analytic sensitivity or produce false-negative results by masking the signal produced by the nucleic acid target. Inclusion of internal controls in PCR-based assays is a valuable strategy not only for monitoring for PCR inhibitors, but also for monitoring nucleic acid extraction efficiency, and for identifying technology errors that may interfere with the ability of an assay to detect the intended target. The Laboratory Technology Committee of the American Association of Veterinary Laboratory Diagnosticians reviewed the different types of internal controls related to monitoring inhibition of PCR-based assays, and provides information here to encourage veterinary diagnostic laboratories to incorporate PCR internal control strategies as a routine quality management component of their molecular testing.
Databáze:	MEDLINE
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