| Autor: |
Burguera EF; Grupo de Investigación en Reumatología (GIR), Agrupación Estratégica CICA-INIBIC, Complexo Hospitalario Universitario A Coruña, Sergas, Instituto de Investigación Biomédica A Coruña-INIBIC, As Xubias 84, 15006, A Coruña, Spain. elena.fernandez.burguera@sergas.es.; CIBER-BBN, Madrid, Spain. elena.fernandez.burguera@sergas.es., Vela-Anero Á; CIBER-BBN, Madrid, Spain.; Grupo de Terapia Celular e Medicina Regenerativa, Departamento de Fisioterapia, Medicina y Ciencias Biomédicas, Instituto de Investigación Biomédica de A Coruña (INIBIC), Complexo Hospitalario Universitario A Coruña, Sergas, Universidad de A Coruña, A Coruña, Spain., Gato-Calvo L; Grupo de Investigación en Reumatología (GIR), Agrupación Estratégica CICA-INIBIC, Complexo Hospitalario Universitario A Coruña, Sergas, Instituto de Investigación Biomédica A Coruña-INIBIC, As Xubias 84, 15006, A Coruña, Spain., Vaamonde-García C; Grupo de Terapia Celular e Medicina Regenerativa, Departamento de Fisioterapia, Medicina y Ciencias Biomédicas, Instituto de Investigación Biomédica de A Coruña (INIBIC), Complexo Hospitalario Universitario A Coruña, Sergas, Universidad de A Coruña, A Coruña, Spain., Meijide-Faílde R; Grupo de Terapia Celular e Medicina Regenerativa, Departamento de Fisioterapia, Medicina y Ciencias Biomédicas, Instituto de Investigación Biomédica de A Coruña (INIBIC), Complexo Hospitalario Universitario A Coruña, Sergas, Universidad de A Coruña, A Coruña, Spain., Blanco FJ; Grupo de Investigación en Reumatología (GIR), Agrupación Estratégica CICA-INIBIC, Complexo Hospitalario Universitario A Coruña, Sergas, Instituto de Investigación Biomédica A Coruña-INIBIC, As Xubias 84, 15006, A Coruña, Spain. fblagar@sergas.es.; Grupo de Investigación en Reumatología (GIR), Departamento de Fisioterapia, Medicina y Ciencias Biomédicas, Instituto de Investigación Biomédica de A Coruña (INIBIC), Complexo Hospitalario Universitario A Coruña, Sergas, Universidad de A Coruña, A Coruña, Spain. fblagar@sergas.es.; ProteoRed/ISCIII, Madrid, Spain. fblagar@sergas.es. |
| Abstrakt: |
Osteoarthritis (OA) is the most common form of arthritis and it is a leading cause of disability in the elderly. Its complete etiology is not known although there are several metabolic, genetic, epigenetic, and local contributing factors involved. At the moment, there is no cure for this pathology and treatment alternatives to retard or stop its progression are intensively being sought. Hydrogen sulfide (H 2 S) is a small gaseous molecule and is present in sulfurous mineral waters as its active component. Data from recent clinical trials shows that balneotherapy (immersion in mineral and/or thermal waters from natural springs) in sulfurous waters can improve OA symptoms, in particular, pain and function. Yet, the underlying mechanisms are poorly known. Hydrogen sulfide is also considered, with NO and CO, an endogenous signaling gasotransmitter. It is synthesized endogenously with the help of three enzymes, cystathionine gamma-lyase (CTH), cystathionine beta-synthase (CBS), and 3-mercaptopyruvate sulfurtransferase (3-MPST). Here, the expression of these three enzymes was demonstrated by quantitative real-time polymerase chain reaction (qRT-PCR) and their protein abundance [by immunohistochemistry and Western blot (WB)] in human articular cartilage. No significant differences were found in CBS or CTH expression or abundance, but mRNA and protein levels of 3-MPST were significantly reduced in cartilage form OA donors. Also, the biosynthesis of H 2 S from OA cartilage, measured with a specific microelectrode, was significantly lower than in OA-free tissue. Yet, no differences were found in H 2 S concentration in serum from OA patients and OA-free donors. The current results suggest that reduced levels of the mitochondrial enzyme 3-MPST in OA cartilage might be, at least in part, responsible for a reduction in H 2 S biosynthesis in this tissue and that impaired H 2 S biosynthesis in the joint might be a contributing factor to OA. This could contribute to explain why exogenous supplementation of H 2 S, for instance with sulfurous thermal water, has positive effects in OA patients. |
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