Role of membrane sterol and redox system in the anti-candida activity reported for Mo-CBP 2 , a protein from Moringa oleifera seeds.

Autor: da Silva Neto JX; Department of Biochemistry and Molecular Biology, Federal University of Ceará, Fortaleza, CE, Brazil., da Costa HPS; Department of Biochemistry and Molecular Biology, Federal University of Ceará, Fortaleza, CE, Brazil., Vasconcelos IM; Department of Biochemistry and Molecular Biology, Federal University of Ceará, Fortaleza, CE, Brazil., Pereira ML; Department of Biology, Federal University of Ceará, Fortaleza, CE, Brazil., Oliveira JTA; Department of Biochemistry and Molecular Biology, Federal University of Ceará, Fortaleza, CE, Brazil., Lopes TDP; Department of Biochemistry and Molecular Biology, Federal University of Ceará, Fortaleza, CE, Brazil., Dias LP; Department of Biochemistry and Molecular Biology, Federal University of Ceará, Fortaleza, CE, Brazil., Araújo NMS; Department of Biochemistry and Molecular Biology, Federal University of Ceará, Fortaleza, CE, Brazil., Moura LFWG; Northeast Biotechnology Network, Graduate Program of Biotechnology, State University of Ceará, Fortaleza, CE, Brazil., Van Tilburg MF; Northeast Biotechnology Network, Graduate Program of Biotechnology, State University of Ceará, Fortaleza, CE, Brazil., Guedes MIF; Northeast Biotechnology Network, Graduate Program of Biotechnology, State University of Ceará, Fortaleza, CE, Brazil., Lopes LA; Department of Biochemistry and Molecular Biology, Federal University of Ceará, Fortaleza, CE, Brazil., Morais EG; Department of Biochemistry and Molecular Biology, Federal University of Ceará, Fortaleza, CE, Brazil., de Oliveira Bezerra de Sousa D; Department of Biochemistry and Molecular Biology, Federal University of Ceará, Fortaleza, CE, Brazil. Electronic address: daniele.sousa@ufc.br.
Jazyk: angličtina
Zdroj: International journal of biological macromolecules [Int J Biol Macromol] 2020 Jan 15; Vol. 143, pp. 814-824. Date of Electronic Publication: 2019 Nov 15.
DOI: 10.1016/j.ijbiomac.2019.09.142
Abstrakt: Plant proteins are emerging as an alternative to conventional treatments against candidiasis. The aim of this study was to better understand the mechanism of action of Mo-CBP 2 against Candida spp, evaluating redox system activity, lipid peroxidation, DNA degradation, cytochrome c release, medium acidification, and membrane interaction. Anti-candida activity of Mo-CBP 2 decreased in the presence of ergosterol, which was not observed with antioxidant agents. C. albicans treated with Mo-CBP 2 also had catalase and peroxidase activities inhibited, while superoxide dismutase was increased. Mo-CBP 2 increased the lipid peroxidation, but it did not alter the ergosterol profile in live cells. External medium acidification was strongly inhibited, and cytochrome c release and DNA degradation were detected. Mo-CBP 2 interacts with cell membrane constituents, changes redox system enzymes in C. albicans and causes lipid peroxidation by ROS overproduction. DNA degradation and cytochrome c release suggest apoptotic or DNAse activity. Lipid peroxidation and H + -ATPases inhibition may induce the process of apoptosis. Finally, Mo-CBP 2 did not have a cytotoxic effect in mammalian Vero cells. This study highlights the biotechnological potential of Mo-CBP 2 as a promising molecule with low toxicity and potent activity. Further studies should be performed to better understand its mode of action and toxicity.
(Copyright © 2019 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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