Emerging strains of porcine epidemic diarrhoea virus (PEDv) in Mexico.

Autor: Reveles-Félix S; Departamento de Medicina y Zootecnia de Cerdos, Facultad de Medicina Veterinaria y Zootecnia (FMVZ), Universidad Nacional Autónoma de México (UNAM), Mexico City, Mexico., Carreón-Nápoles R; Departamento de Medicina y Zootecnia de Cerdos, Facultad de Medicina Veterinaria y Zootecnia (FMVZ), Universidad Nacional Autónoma de México (UNAM), Mexico City, Mexico., Mendoza-Elvira S; Laboratorio de Virología, Facultad de Estudios Superiores Cuautitlán (FESC), UNAM, Cuautitlan Izcalli, Mexico., Quintero-Ramírez V; Departamento de Patología, FESC-UNAM, Cuautitlan Izcalli, Mexico., García-Sánchez J; Departamento de Medicina y Zootecnia de Cerdos, Facultad de Medicina Veterinaria y Zootecnia (FMVZ), Universidad Nacional Autónoma de México (UNAM), Mexico City, Mexico., Martínez-Bautista R; Departamento de Medicina y Zootecnia de Cerdos, Facultad de Medicina Veterinaria y Zootecnia (FMVZ), Universidad Nacional Autónoma de México (UNAM), Mexico City, Mexico., Saavedra-Montañez M; Departamento de Microbiología e Inmunología, FMVZ-UNAM, Mexico City, Mexico., Mosqueda Gualito JJ; Universidad Autonoma de Querétaro, Cerro de las campanas, Querétaro, Mexico., Sánchez-Betancourt JI; Departamento de Medicina y Zootecnia de Cerdos, Facultad de Medicina Veterinaria y Zootecnia (FMVZ), Universidad Nacional Autónoma de México (UNAM), Mexico City, Mexico.
Jazyk: angličtina
Zdroj: Transboundary and emerging diseases [Transbound Emerg Dis] 2020 Mar; Vol. 67 (2), pp. 1035-1041. Date of Electronic Publication: 2019 Dec 16.
DOI: 10.1111/tbed.13426
Abstrakt: As an emerging disease, the porcine epidemic diarrhoea virus has caused substantial economic losses to the pork industry in Mexico, leading to piglet mortality rates of up to 100%. For detection, sequencing and genetic characterization of the virus, 68 samples of one-week-old piglets from pork farms in 17 states of Mexico were analysed. In total, 53 samples were positive by real-time RT-PCR, confirming the presence of the virus in 15 states. Twenty-eight samples from 10 states were amplified by endpoint RT-PCR, and 20 sequences of the spike gene were obtained. A phylogenetic analysis based on the spike gene demonstrated that all Mexican strains are in Group II and are classified as non-Indel-S emerging variants. Three strains showed amino acid insertions: PEDv/MEX/GTO/LI-DMZC15/2015 and PEDv/MEX/QRO/LI-DMZC45/2016 showed one amino acid insertion ( 424 Y 425 and 447 D 448 , respectively), and PEDv/MEX/QRO/LI-DMZC49/2019 showed one and two amino acid insertions ( 422 C 423 and 537 SQ 538 ), with the second insertion in the COE region. These results provide evidence of the prevalence of emerging, non-Indel-S strains of the virus are currently circulating in Mexico during 2016-2018, when three of which have amino acid insertions: PEDv/MEX/GTO/IN-DMZC15/2015 and PEDv/MEX/QRO/IN-DMZC45/2016 have one amino acid insertion each ( 424 Y 425 and 447 D 448 , respectively), and PEDv/MEX/QRO/IN-DMZC49/2019 has one ( 422 C 423 ) and two amino acid insertions ( 537 SQ 538 ), the latter being in the COE region, which could generate new antigenic variants.
(© 2019 Blackwell Verlag GmbH.)
Databáze: MEDLINE
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