"Fishing and Hunting"-Selective Immobilization of a Recombinant Phenylalanine Ammonia-Lyase from Fermentation Media.

Autor: Sánta-Bell E; Department of Organic Chemistry and Technology, Budapest University of Technology and Economics,1111 Budapest, Hungary., Molnár Z; Department of Organic Chemistry and Technology, Budapest University of Technology and Economics,1111 Budapest, Hungary.; Fermentia Microbiological Ltd., 1405 Budapest, Hungary.; Institute of Enzymology, Research Center for Natural Sciences, Hungarian Academy of Science,1117 Budapest, Hungary., Varga A; Biocatalysis and Biotransformation Research Centre, Faculty of Chemistry and Chemical Engineering,Babeş-Bolyai University of Cluj-Napoca, 400028 Cluj-Napoca, Romania., Nagy F; Department of Organic Chemistry and Technology, Budapest University of Technology and Economics,1111 Budapest, Hungary., Hornyánszky G; Department of Organic Chemistry and Technology, Budapest University of Technology and Economics,1111 Budapest, Hungary.; SynBiocat Ltd., 1172 Budapest, Hungary., Paizs C; Biocatalysis and Biotransformation Research Centre, Faculty of Chemistry and Chemical Engineering,Babeş-Bolyai University of Cluj-Napoca, 400028 Cluj-Napoca, Romania., Balogh-Weiser D; Department of Organic Chemistry and Technology, Budapest University of Technology and Economics,1111 Budapest, Hungary.; SynBiocat Ltd., 1172 Budapest, Hungary.; Department of Physical Chemistry and Materials Science, Budapest University of Technology and Economics, 1111 Budapest, Hungary., Poppe L; Department of Organic Chemistry and Technology, Budapest University of Technology and Economics,1111 Budapest, Hungary.; Biocatalysis and Biotransformation Research Centre, Faculty of Chemistry and Chemical Engineering,Babeş-Bolyai University of Cluj-Napoca, 400028 Cluj-Napoca, Romania.; SynBiocat Ltd., 1172 Budapest, Hungary.
Jazyk: angličtina
Zdroj: Molecules (Basel, Switzerland) [Molecules] 2019 Nov 15; Vol. 24 (22). Date of Electronic Publication: 2019 Nov 15.
DOI: 10.3390/molecules24224146
Abstrakt: This article overviews the numerous immobilization methods available for various biocatalysts such as whole-cells, cell fragments, lysates or enzymes which do not require preliminary enzyme purification and introduces an advanced approach avoiding the costly and time consuming downstream processes required by immobilization of purified enzyme-based biocatalysts (such as enzyme purification by chromatographic methods and dialysis). Our approach is based on silica shell coated magnetic nanoparticles as solid carriers decorated with mixed functions having either coordinative binding ability (a metal ion complexed by a chelator anchored to the surface) or covalent bond-forming ability (an epoxide attached to the surface via a proper linker) enabling a single operation enrichment and immobilization of a recombinant phenylalanine ammonia-lyase from parsley fused to a polyhistidine affinity tag.
Competing Interests: SynBiocat Ltd. has interest in commercialization of supports for enzyme immobilization and immobilized biocatalysts. Besides that, the authors declare no conflict of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript, or in the decision to publish the results.
Databáze: MEDLINE
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