Telomere-Mediated Chromosomal Truncation for Generating Engineered Minichromosomes in Maize.

Autor: Swyers NC; Division of Biological Sciences, University of Missouri, Columbia, Missouri., Cody JP; Division of Biological Sciences, University of Missouri, Columbia, Missouri., McCaw ME; Division of Biological Sciences, University of Missouri, Columbia, Missouri., Graham ND; Division of Biological Sciences, University of Missouri, Columbia, Missouri., Zhao C; Division of Biological Sciences, University of Missouri, Columbia, Missouri., Gaeta RT; Division of Biological Sciences, University of Missouri, Columbia, Missouri., Birchler JA; Division of Biological Sciences, University of Missouri, Columbia, Missouri.
Jazyk: angličtina
Zdroj: Current protocols in plant biology [Curr Protoc Plant Biol] 2016 Sep; Vol. 1 (3), pp. 488-500.
DOI: 10.1002/cppb.20031
Abstrakt: Minichromosomes have been generated in maize using telomere-mediated truncation. Telomere DNA, because of its repetitive nature, can be difficult to manipulate. The protocols in this unit describe two methods for generating the telomere DNA required for the initiation of telomere-mediated truncation. The resulting DNA can then be used with truncation cassettes for introduction into maize via transformation. © 2016 by John Wiley & Sons, Inc.
(Copyright © 2016 John Wiley & Sons, Inc.)
Databáze: MEDLINE
Externí odkaz: