Continual Conscious Bioluminescent Imaging in Freely Moving Mice.

Autor:	Diaz JA; Gene Transfer Technology Group, Institute for Women's Health, University College London, London, UK., Geard A; UCL School of Pharmacy, University College London, London, UK.; Wits/SAMRC Antiviral Gene Therapy Research Unit, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa., FitzPatrick LM; Centre for Biomedicine, Manchester Metropolitan University, Manchester, UK., Delhove JMKM; Robinson Research Institute, University of Adelaide, Adelaide, SA, Australia., Buckley SMK; Gene Transfer Technology Group, Institute for Women's Health, University College London, London, UK., Waddington SN; Gene Transfer Technology Group, Institute for Women's Health, University College London, London, UK.; Wits/SAMRC Antiviral Gene Therapy Research Unit, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa., McKay TR; Centre for Biomedicine, Manchester Metropolitan University, Manchester, UK., Karda R; Gene Transfer Technology Group, Institute for Women's Health, University College London, London, UK. r.karda@ucl.ac.uk.
Jazyk:	angličtina
Zdroj:	Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2020; Vol. 2081, pp. 161-175.
DOI:	10.1007/978-1-4939-9940-8_11
Abstrakt:	In vivo bioluminescent imaging allows the detection of reporter gene expression in rodents in real time. Here we describe a novel technology whereby we can generate somatotransgenic rodents with the use of a viral vector carrying a luciferase transgene. We are able to achieve long term luciferase expression by a single injection of lentiviral or adeno-associated virus vectors to newborn mice. Further, we describe whole body bioluminescence imaging of conscious mice in a noninvasive manner, thus enforcing the 3R's (replacement, reduction, and refinement) of biomedical animal research.
Databáze:	MEDLINE
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