Dual Regulation of L-Selectin-Mediated Leukocyte Adhesion by Endothelial Surface Glycocalyx.

Autor: Dragovich MA; 1Department of Mechanical Engineering & Mechanics, Lehigh University, 19 Memorial Drive West, Bethlehem, PA 18015 USA., Genemaras K; 2Bioengineering Program, Lehigh University, 19 Memorial Drive West, Bethlehem, PA 18015 USA.; 4Present Address: Bioinnovation Program, Tulane University, 6823 Saint Charles Avenue, New Orleans, LA 70118 USA., Dailey HL; 1Department of Mechanical Engineering & Mechanics, Lehigh University, 19 Memorial Drive West, Bethlehem, PA 18015 USA., Jedlicka S; 2Bioengineering Program, Lehigh University, 19 Memorial Drive West, Bethlehem, PA 18015 USA.; 3Department of Materials Science and Engineering, Lehigh University, 19 Memorial Drive West, Bethlehem, PA 18015 USA., Frank Zhang X; 1Department of Mechanical Engineering & Mechanics, Lehigh University, 19 Memorial Drive West, Bethlehem, PA 18015 USA.; 2Bioengineering Program, Lehigh University, 19 Memorial Drive West, Bethlehem, PA 18015 USA.
Jazyk: angličtina
Zdroj: Cellular and molecular bioengineering [Cell Mol Bioeng] 2016 Aug 25; Vol. 10 (1), pp. 102-113. Date of Electronic Publication: 2016 Aug 25 (Print Publication: 2017).
DOI: 10.1007/s12195-016-0463-6
Abstrakt: Endothelial surface glycocalyx (ESG) is a carbohydrate-rich, gel-like layer found on vascular endothelium, serving critical functions in mechanotransduction of blood flows, maintenance of the endothelial permeability, and the control of leukocyte adhesion and inflammation. This study aimed to clarify the role of ESG in the adhesion between leukocytes and Human Umbilical Vein Endothelial Cells (HUVECs) under resting or inflammatory conditions. Using an atomic force microscopy-based single-cell adhesion assay, we directly quantified the detachment force and work perpendicular to the cell membrane. Detachment force and work were measured for every separation event of a leukocyte from a HUVEC with ESG, or with the major ESG glycosaminoglycan components, heparan sulfate (HS) and hyaluronic acid (HA) removed. For the resting HUVECs, when HS and/or HA were removed, the detachment force and work increased dramatically. For the HUVECs activated by inflammatory cytokine tumor necrosis factor alpha, we observed increases in the detachment force and work compared to the resting HUVECs, and removal of HS and/or HA resulted in significant decreases in the detachment force and work. The results demonstrate that the ESG layer serves a dual function: (1) on resting endothelium, it prevents leukocyte adhesion, and (2) under inflammatory conditions, it participates in endothelial-leukocyte interactions with molecules such as selectins.
(© Biomedical Engineering Society 2016.)
Databáze: MEDLINE
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