SeeTB: A novel alternative to sputum smear microscopy to diagnose tuberculosis in high burden countries.

Autor: Pandey V; Indian Institute of Technology, New Delhi, 110016, India.; Valetude Primus Healthcare Pvt Ltd, Okhla Phase 2, New Delhi, 110020, India., Singh P; Indian Institute of Technology, New Delhi, 110016, India., Singh S; Indian Institute of Technology, New Delhi, 110016, India., Arora N; Indian Institute of Technology, New Delhi, 110016, India., Quadir N; National Institute of Pathology, Safdarjung Hospital, New Delhi, 110029, India., Singh S; Indian Institute of Technology, New Delhi, 110016, India.; Valetude Primus Healthcare Pvt Ltd, Okhla Phase 2, New Delhi, 110020, India., Das A; Jamia Hamdard, Hamdard Nagar, New Delhi, 110062, India., Dudeja M; Jamia Hamdard, Hamdard Nagar, New Delhi, 110062, India., Kapur P; Jamia Hamdard, Hamdard Nagar, New Delhi, 110062, India., Ehtesham NZ; National Institute of Pathology, Safdarjung Hospital, New Delhi, 110029, India., Elangovan R; Indian Institute of Technology, New Delhi, 110016, India. rke.iitd@gmail.com.; Valetude Primus Healthcare Pvt Ltd, Okhla Phase 2, New Delhi, 110020, India. rke.iitd@gmail.com., Hasnain SE; Indian Institute of Technology, New Delhi, 110016, India. sehiitd@gmail.com.; Valetude Primus Healthcare Pvt Ltd, Okhla Phase 2, New Delhi, 110020, India. sehiitd@gmail.com.; Jamia Hamdard, Hamdard Nagar, New Delhi, 110062, India. sehiitd@gmail.com.; DRILS, University of Hyderabad Campus, Prof C.R. Rao Road, Hyderabad, 500046, India. sehiitd@gmail.com.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2019 Nov 12; Vol. 9 (1), pp. 16371. Date of Electronic Publication: 2019 Nov 12.
DOI: 10.1038/s41598-019-52739-9
Abstrakt: Microscopy-based tuberculosis (TB) diagnosis i.e. Ziehl-Neelsen screening still remains the primary diagnostic method in resource poor and high TB burden countries, however this method has poor sensitivity (~60%). Bringing three million TB patients who are left undiagnosed under the treatment has been a major focus as part of END-TB strategy across the world. We have developed a portable set-up called 'SeeTB' that converts a bright-field microscope into fluorescence microscope (FM) with minimal interventions. SeeTB, a total internal reflection-based fluorescence excitation system allows visualization of auramine-O stained bacilli efficiently with high signal-to-noise ratio. Along with the device, we have developed a sputum-processing reagent called 'CLR' that homogenizes and digests the viscous polymer matrix of sputum. We have compared the performance of SeeTB system in 237 clinical sputum samples along with FM, GeneXpert and liquid culture. In comparison with culture as gold standard, FM has sensitivity of 63.77% and SeeTB has improved sensitivity to 76.06%. In comparison with GeneXpert, FM has sensitivity of 73.91% while SeeTB has improved sensitivity to 85.51%. However, there is no significant change in the specificity between FM and SeeTB system. In short, SeeTB system offers the most realistic option for improved TB case identification in resource-limited settings.
Databáze: MEDLINE
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