A full semester flow cytometry course improves graduate and undergraduate student confidence.

Autor: Tueller JA; Department of Microbiology and Molecular Biology, Brigham Young University, Provo, Utah., Whitley KV; Department of Microbiology and Molecular Biology, Brigham Young University, Provo, Utah., Weber KS; Department of Microbiology and Molecular Biology, Brigham Young University, Provo, Utah.
Jazyk: angličtina
Zdroj: Biochemistry and molecular biology education : a bimonthly publication of the International Union of Biochemistry and Molecular Biology [Biochem Mol Biol Educ] 2020 Mar; Vol. 48 (2), pp. 99-107. Date of Electronic Publication: 2019 Nov 12.
DOI: 10.1002/bmb.21318
Abstrakt: Flow cytometry is a versatile and high throughput technique for rapid and efficient biological testing. It requires a high level of conceptual, technical, and analytical skills to properly design experiments, effectively operate flow cytometry machines, and analyze the data. A lack of training and development of any of these three skills can result in underutilization and improper use of flow cytometric machines that can impede research progress. Often students develop these conceptual, technical, and analysis skills from trial and error, but many students either do not use this powerful flow cytometry technology, use it improperly or ineffectively, or give up using it without proper training and support. Here we report on a course which teaches flow cytometry skills to undergraduate and graduate students. The design of this course is unique in that it teaches conceptual, technical, and analytical skills related to flow cytometry in a full semester format. Undergraduate and graduate students reported significant increases in their confidence levels over the course of the semester. Here we provide our findings and resources for others who may want to implement a similar course.
(© 2019 International Union of Biochemistry and Molecular Biology.)
Databáze: MEDLINE
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