Evidence for a transcellular route for vitellogenin transport in the telotrophic ovary of Podisus nigrispinus (Hemiptera: Pentatomidae).

Autor: Assis MQ; Department of General Biology, Universidade Federal de Viçosa, 36570-000, Viçosa, Brazil., Dohanik VT; Department of General Biology, Universidade Federal de Viçosa, 36570-000, Viçosa, Brazil., Oliveira LL; Department of General Biology, Universidade Federal de Viçosa, 36570-000, Viçosa, Brazil., Zanuncio JC; Departament of Entomology, Universidade Federal de Viçosa, 36570-000, Viçosa, Brazil., Serrão JE; Department of General Biology, Universidade Federal de Viçosa, 36570-000, Viçosa, Brazil. jeserrao@ufv.br.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2019 Nov 11; Vol. 9 (1), pp. 16441. Date of Electronic Publication: 2019 Nov 11.
DOI: 10.1038/s41598-019-52789-z
Abstrakt: Vitellogenin is the main yolk precursor protein in insect oocytes. It is synthesized in the fat body and released into the hemolymph. To reach the oocyte surface, vitellogenin must cross a single layer of follicular epithelium cells. The transport of vitellogenin across the follicular epithelium has been suggested to occur through the enlarged intercellular spaces (patency) by a paracellular route or by endocytosis by follicular cells and release onto oocyte surface in a transcelluar route. In this study, we investigated whether vitellogenin transport in the meroistic telotrophic ovary of Podisus nigrispinus (Hemiptera) occurs via a paracellular or transcellular route. Light and transmission electron microscopies showed that short cell-cell contacts with well-developed occluding septate junctions were present in follicular cells with patency. Immunofluorescence microscopy revealed the presence of vitellogenin receptors in the plasma membrane and of vitellogenin in the cytoplasm of follicular cells. Data suggest that cell-cell contacts serve as a barrier to large vitellogenin molecules and that this protein is transported via a transcellular route of receptor-mediated endocytosis.
Databáze: MEDLINE
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