Use of the natural products from the leaves of the fruitfull tree Persea americana against Candida sp. biofilms using acrylic resin discs.
Autor: | de Freitas MA; Laboratory of Medical Mycology Sylvio Campos, Department of Mycology, Federal University of Pernambuco-UFPE, Recife, PE, Brazil., Andrade JC; Laboratory of Microbiology and Molecular Biology, Department of Biological Chemistry, Regional University of Cariri - URCA, Crato, CE, Brazil., Alves AIS; Laboratory of Medical Mycology Sylvio Campos, Department of Mycology, Federal University of Pernambuco-UFPE, Recife, PE, Brazil., Dos Santos FAG; Laboratory of Medical Mycology Sylvio Campos, Department of Mycology, Federal University of Pernambuco-UFPE, Recife, PE, Brazil., Leite-Andrade MC; Laboratory of Medical Mycology Sylvio Campos, Department of Mycology, Federal University of Pernambuco-UFPE, Recife, PE, Brazil., Sales DL; Laboratory of Microbiology and Molecular Biology, Department of Biological Chemistry, Regional University of Cariri - URCA, Crato, CE, Brazil., Nunes M; Laboratory of Medical Mycology Sylvio Campos, Department of Mycology, Federal University of Pernambuco-UFPE, Recife, PE, Brazil., Ribeiro PRV; Multiuser Laboratory of Natural Products Chemistry LMQPN, Embrapa Agroindustria Tropical, Fortaleza, CE, Brazil., Melo Coutinho HD; Laboratory of Microbiology and Molecular Biology, Department of Biological Chemistry, Regional University of Cariri - URCA, Crato, CE, Brazil. Electronic address: hdmcoutinho@urca.br., Morais-Braga MFB; Laboratory of Microbiology and Molecular Biology, Department of Biological Chemistry, Regional University of Cariri - URCA, Crato, CE, Brazil., Neves RP; Laboratory of Medical Mycology Sylvio Campos, Department of Mycology, Federal University of Pernambuco-UFPE, Recife, PE, Brazil. |
---|---|
Jazyk: | angličtina |
Zdroj: | The Science of the total environment [Sci Total Environ] 2020 Feb 10; Vol. 703, pp. 134779. Date of Electronic Publication: 2019 Nov 02. |
DOI: | 10.1016/j.scitotenv.2019.134779 |
Abstrakt: | The search for natural substances such as plant extracts with antimicrobial properties has considerably increased, given that biofilms constitute a barrier against antifungal therapy, where these can be formed on any surface, such as acrylic resin prosthesis. The objective of this study was to identify the chemical composition of the Persea americana Mill. leaf ethanol extract (EEFPa) using the UPLC-QTOF-MS/MS technique, to verify its antifungal activity through a sensitivity test according to the conditions described in the documents in M27-A3 (CLSI, 2008) and M60 (CLSI, 2017), to induce biofilm formation in acrylic resin discs and quantify their formation using tetrazolium salt reduction (MTT), as well as to treat these with the extract and fluconazole. Ten of the twelve compounds present in the extract were identified. In the sensitivity test the lowest minimum inhibitory concentration observed was 512 μg/mL, while fluconazole concentrations ranged from 64 to 1 μg/mL. During biofilm induction, all the isolates were able to form biofilms within 48 h. During biofilm treatment, the extract was less effective at biofilm reduction than Fluconazole. The EEFPa showed significant antifungal activity against some of the strains in this study, however the extract showed lower effect when compared to fluconazole against the biofilm formation. (Copyright © 2019 Elsevier B.V. All rights reserved.) |
Databáze: | MEDLINE |
Externí odkaz: |
načítá se...