Simple Surgical Induction of Conductive Hearing Loss with Verification Using Otoscope Visualization and Behavioral Clap Startle Response in Rat.

Autor:	Manno FAM; Instituto de Neurobiología, Universidad Nacional Autónoma de México, México., Manno SHC; State Key Laboratory of Marine Pollution (SKLMP), City University of Hong Kong, Hong Kong; Department of Biomedical Sciences, College of Veterinary Medicine and Life Sciences, City University of Hong Kong, Hong Kong; Department of Physics, City University of Hong Kong, Hong Kong., Ma V; Department of Clinical Oncology, Queen Elizabeth Hospital, Hong Kong., Barrios FA; Instituto de Neurobiología, Universidad Nacional Autónoma de México, México., Cho WC; Department of Clinical Oncology, Queen Elizabeth Hospital, Hong Kong., Cheng SH; State Key Laboratory of Marine Pollution (SKLMP), City University of Hong Kong, Hong Kong; Department of Biomedical Sciences, College of Veterinary Medicine and Life Sciences, City University of Hong Kong, Hong Kong; Department of Materials Science and Engineering, City University of Hong Kong, Hong Kong., Lau C; Department of Physics, City University of Hong Kong, Hong Kong; condon.lau@cityu.edu.hk.
Jazyk:	angličtina
Zdroj:	Journal of visualized experiments : JoVE [J Vis Exp] 2019 Oct 26 (152). Date of Electronic Publication: 2019 Oct 26.
DOI:	10.3791/57993
Abstrakt:	Conductive hearing loss (CHL) is a prevalent hearing impairment in humans. The goal of the protocol is to describe a simple surgical procedure for inducing CHL in rodents. The protocol demonstrates CHL by tympanic membrane puncture. Verification of CHL surgery was by otoscope examination and behavioral assessment by clap startle response, both replicable and reliable, and are simple methods to demonstrate hearing loss has occurred. The simple CHL procedure is advantageous due to its reproducibility and flexibility to different pursuits in hearing loss research. The limitations of inducing CHL by a surgical approach are associated with the learning curve to perform the surgical procedure and confidence in audiological examination. Inducing a hearing impairment by CHL allows one to readily study the neural manifestations and behavioral outcomes of hearing loss.
Databáze:	MEDLINE
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