Immunological observations and transcriptomic analysis of trimester-specific full-term placentas from three Zika virus-infected women.

Autor: Lum FM; Singapore Immunology Network Agency for Science, Technology and Research Singapore Singapore., Narang V; Singapore Immunology Network Agency for Science, Technology and Research Singapore Singapore., Hue S; Institute of Molecular and Cell Biology Agency for Science, Technology and Research Singapore Singapore.; Department of Pathology National University Health System Singapore Singapore., Chen J; KK Women's and Children's Hospital Singapore Singapore., McGovern N; Department of Pathology University of Cambridge Cambridge UK., Rajarethinam R; Institute of Molecular and Cell Biology Agency for Science, Technology and Research Singapore Singapore., Tan JJ; Singapore Immunology Network Agency for Science, Technology and Research Singapore Singapore., Amrun SN; Singapore Immunology Network Agency for Science, Technology and Research Singapore Singapore., Chan YH; Singapore Immunology Network Agency for Science, Technology and Research Singapore Singapore.; NUS Graduate School for Integrative Sciences and Engineering National University of Singapore Singapore Singapore., Lee CY; Singapore Immunology Network Agency for Science, Technology and Research Singapore Singapore.; NUS Graduate School for Integrative Sciences and Engineering National University of Singapore Singapore Singapore., Chua TK; Singapore Immunology Network Agency for Science, Technology and Research Singapore Singapore., Yee WX; Singapore Immunology Network Agency for Science, Technology and Research Singapore Singapore., Yeo NK; Singapore Immunology Network Agency for Science, Technology and Research Singapore Singapore., Tan TC; Department of O&G KK Women's and Children's Hospital Singapore Singapore., Liu X; Centre for Genomic Research Institute of Integrative Biology University of Liverpool Liverpool UK.; NIHR Health Protection Research Unit in Emerging and Zoonotic Infections University of Liverpool Liverpool UK., Haldenby S; Centre for Genomic Research Institute of Integrative Biology University of Liverpool Liverpool UK., Leo YS; Communicable Diseases Centre Institute of Infectious Diseases and Epidemiology Tan Tock Seng Hospital Singapore Singapore.; National Centre for Infectious Diseases Singapore Singapore.; Saw Swee Hock School of Public Health National University of Singapore Singapore Singapore.; Lee Kong Chian School of Medicine Nanyang Technological University Singapore Singapore., Ginhoux F; Singapore Immunology Network Agency for Science, Technology and Research Singapore Singapore., Chan JK; Singapore Immunology Network Agency for Science, Technology and Research Singapore Singapore.; Department of Reproductive Medicine KK Women's and Children's Hospital Singapore Singapore.; KK Research Centre KK Women's and Children's Hospital Singapore Singapore.; Cancer and Stem Cell Program Duke-NUS Medical School Singapore Singapore., Hiscox J; Singapore Immunology Network Agency for Science, Technology and Research Singapore Singapore.; NIHR Health Protection Research Unit in Emerging and Zoonotic Infections University of Liverpool Liverpool UK.; Institute of Infection and Global Health University of Liverpool Liverpool UK., Chong CY; Department of Paediatrics KK Women's and Children's Hospital Singapore Singapore.; Lee Kong Chian School of Medicine Nanyang Technological University Singapore Singapore.; Yong Loo Lin School of Medicine National University of Singapore Singapore Singapore.; Graduate Medical School Duke-NUS Medical School Singapore Singapore., Ng LF; Singapore Immunology Network Agency for Science, Technology and Research Singapore Singapore.; NIHR Health Protection Research Unit in Emerging and Zoonotic Infections University of Liverpool Liverpool UK.; Institute of Infection and Global Health University of Liverpool Liverpool UK.; Department of Biochemistry Yong Loo Lin School of Medicine National University of Singapore Singapore Singapore.
Jazyk: angličtina
Zdroj: Clinical & translational immunology [Clin Transl Immunology] 2019 Nov 05; Vol. 8 (11), pp. e01082. Date of Electronic Publication: 2019 Nov 05 (Print Publication: 2019).
DOI: 10.1002/cti2.1082
Abstrakt: Objectives: Effects of Zika virus (ZIKV) infection on placental development during pregnancy are unclear.
Methods: Full-term placentas from three women, each infected with ZIKV during specific pregnancy trimesters, were harvested for anatomic, immunologic and transcriptomic analysis.
Results: In this study, each woman exhibited a unique immune response with raised IL-1RA, IP-10, EGF and RANTES expression and neutrophil numbers during the acute infection phase. Although ZIKV NS3 antigens co-localised to placental Hofbauer cells, the placentas showed no anatomic defects. Transcriptomic analysis of samples from the placentas revealed that infection during trimester 1 caused a disparate cellular response centred on differential eIF2 signalling, mitochondrial dysfunction and oxidative phosphorylation. Despite these, the babies were delivered without any congenital anomalies.
Conclusion: These findings should translate to improve clinical prenatal screening procedures for virus-infected pregnant patients.
Competing Interests: The authors declare no conflict of interest.
(© 2019 The Authors. Clinical & Translational Immunology published by John Wiley & Sons Australia, Ltd on behalf of Australian and New Zealand Society for Immunology Inc.)
Databáze: MEDLINE
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