Putative Mixotrophic Nitrifying-Denitrifying Gammaproteobacteria Implicated in Nitrogen Cycling Within the Ammonia/Oxygen Transition Zone of an Oil Sands Pit Lake.
Autor: | Mori JF; Department of Civil and Mineral Engineering, University of Toronto, Toronto, ON, Canada., Chen LX; Department of Earth and Planetary Sciences, University of California, Berkeley, Berkeley, CA, United States., Jessen GL; Department of Civil and Mineral Engineering, University of Toronto, Toronto, ON, Canada., Rudderham SB; Department of Geological Sciences, University of Saskatchewan, Saskatoon, SK, Canada., McBeth JM; Department of Geological Sciences, University of Saskatchewan, Saskatoon, SK, Canada., Lindsay MBJ; Department of Geological Sciences, University of Saskatchewan, Saskatoon, SK, Canada., Slater GF; School of Geography and Earth Science, McMaster University, Hamilton, ON, Canada., Banfield JF; Department of Earth and Planetary Sciences, University of California, Berkeley, Berkeley, CA, United States., Warren LA; Department of Civil and Mineral Engineering, University of Toronto, Toronto, ON, Canada.; School of Geography and Earth Science, McMaster University, Hamilton, ON, Canada. |
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Jazyk: | angličtina |
Zdroj: | Frontiers in microbiology [Front Microbiol] 2019 Oct 24; Vol. 10, pp. 2435. Date of Electronic Publication: 2019 Oct 24 (Print Publication: 2019). |
DOI: | 10.3389/fmicb.2019.02435 |
Abstrakt: | Anthropogenically-impacted environments offer the opportunity to discover novel microbial species and metabolisms, which may be undetectable in natural systems. Here, a combined metagenomic and geochemical study in Base Mine Lake, Alberta, Canada, which is the only oil sands end pit lake to date, revealed that nitrification was performed by members from Nitrosomonadaceae, Chloroflexi and unclassified Gammaproteobacteria "MBAE14." While Nitrosomonadaceae and Chloroflexi groups were relatively abundant in the upper oxygenated zones, MBAE14 dominated the hypoxic hypolimnetic zones (approximately 30% of total microbial communities); MBAE14 was not detected in the underlying anoxic tailings. Replication rate analyses indicate that MBAE14 grew in metalimnetic and hypolimnetic water cap regions, most actively at the metalimnetic, ammonia/oxygen transition zone consistent with it putatively conducting nitrification. Detailed genomic analyses of MBAE14 evidenced both ammonia oxidation and denitrification into dinitrogen capabilities. However, the absence of known CO (Copyright © 2019 Mori, Chen, Jessen, Rudderham, McBeth, Lindsay, Slater, Banfield and Warren.) |
Databáze: | MEDLINE |
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