In vitro nitazoxanide exposure affects energetic metabolism of Taenia crassiceps.

Autor: Isac E; Tropical Pathology and Public Health Institute, Federal University of Goias, Goiás, Rua 235, S/n, Setor Leste Universitário, CEP 74605-050, Brazil., Picanço GA; Tropical Pathology and Public Health Institute, Federal University of Goias, Goiás, Rua 235, S/n, Setor Leste Universitário, CEP 74605-050, Brazil., Costa TL; Tropical Pathology and Public Health Institute, Federal University of Goias, Goiás, Rua 235, S/n, Setor Leste Universitário, CEP 74605-050, Brazil., Lima NF; Tropical Pathology and Public Health Institute, Federal University of Goias, Goiás, Rua 235, S/n, Setor Leste Universitário, CEP 74605-050, Brazil., Alves DSMM; Tropical Pathology and Public Health Institute, Federal University of Goias, Goiás, Rua 235, S/n, Setor Leste Universitário, CEP 74605-050, Brazil., Fraga CM; Tropical Pathology and Public Health Institute, Federal University of Goias, Goiás, Rua 235, S/n, Setor Leste Universitário, CEP 74605-050, Brazil., Lino Junior RS; Tropical Pathology and Public Health Institute, Federal University of Goias, Goiás, Rua 235, S/n, Setor Leste Universitário, CEP 74605-050, Brazil., Vinaud MC; Tropical Pathology and Public Health Institute, Federal University of Goias, Goiás, Rua 235, S/n, Setor Leste Universitário, CEP 74605-050, Brazil. Electronic address: marinavinaud@gmail.com.
Jazyk: angličtina
Zdroj: Experimental parasitology [Exp Parasitol] 2020 Jan; Vol. 208, pp. 107792. Date of Electronic Publication: 2019 Nov 07.
DOI: 10.1016/j.exppara.2019.107792
Abstrakt: Nitazoxanide (NTZ) is a broad-spectrum drug used in intestinal infections, but still poorly explored in the treatment of parasitic tissular infections. This study aimed to evaluate the in vitro responses of the energetic metabolism of T. crassiceps cysticerci induced by NTZ. The organic acids of the tricarboxylic acid cycle, products derived from fatty acids oxidation and protein catabolism were analyzed. These acids were quantified after 24 h of in vitro exposure to different NTZ concentrations. A positive control group was performed with albendazole sulfoxide (ABZSO). The significant alterations in citrate, fumarate and malate concentrations showed the NTZ influence in the tricarboxylic acid (TCA) cycle. The non-detection of acetate confirmed that the main mode of action of NTZ is effective against T. crassiceps cysticerci. The statistical differences in fumarate, urea and beta-hydroxybutyrate concentrations showed the NTZ effect on protein catabolism and fatty acid oxidation. Therefore, the main energetic pathways such as the TCA cycle, protein catabolism and fatty acids oxidation were altered after in vitro NTZ exposure. In conclusion, NTZ induced a significant metabolic stress in the parasite indicating that it may be used as an alternative therapeutic choice for cysticercosis treatment. The use of metabolic approaches to establish comparisons between anti parasitic drugs mode of actions is proposed.
(Copyright © 2019 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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