Humoral Immunogenicity and Efficacy of a Single Dose of ChAdOx1 MERS Vaccine Candidate in Dromedary Camels.

Autor: Alharbi NK; Department of Infectious Disease Research, King Abdullah International Medical Research Center, Riyadh, Saudi Arabia. harbina2@ngha.med.sa.; King Saud bin Abdulaziz University for Health Sciences, Riyadh, Saudi Arabia. harbina2@ngha.med.sa., Qasim I; Ministry of Environment, Water and Agriculture (MEWA), Riyadh, Saudi Arabia., Almasoud A; Department of Infectious Disease Research, King Abdullah International Medical Research Center, Riyadh, Saudi Arabia., Aljami HA; Department of Infectious Disease Research, King Abdullah International Medical Research Center, Riyadh, Saudi Arabia., Alenazi MW; Department of Infectious Disease Research, King Abdullah International Medical Research Center, Riyadh, Saudi Arabia., Alhafufi A; Ministry of Environment, Water and Agriculture (MEWA), Riyadh, Saudi Arabia., Aldibasi OS; Department of Bioinformatics and Biostatistics, King Abdullah International Medical Research Center, Riyadh, Saudi Arabia., Hashem AM; Department of Medical Microbiology and Parasitology, Faculty of Medicine, King Abdulaziz University, Jeddah, Saudi Arabia.; Vaccines and Immunotherapy Unit, King Fahd Medical Research Center, King Abdulaziz University, Jeddah, Saudi Arabia., Kasem S; Ministry of Environment, Water and Agriculture (MEWA), Riyadh, Saudi Arabia.; Department of Virology, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafrelsheikh, El Geish Street, 33516, Egypt., Albrahim R; Ministry of Environment, Water and Agriculture (MEWA), Riyadh, Saudi Arabia., Aldubaib M; College of Agriculture and Veterinary Medicine, Qassim University, Qassim, Saudi Arabia., Almansour A; Ministry of Environment, Water and Agriculture (MEWA), Riyadh, Saudi Arabia., Temperton NJ; Viral Pseudotype Unit, Medway School of Pharmacy, University of Kent, Chatham, Kent, ME4 4TB, United Kingdom., Kupke A; Institute of Virology, Philipps University of Marburg, Marburg, Germany.; German Center for Infection Research (DZIF), Partner Site Gieβen-Marburg-Langen, Germany., Becker S; Institute of Virology, Philipps University of Marburg, Marburg, Germany.; German Center for Infection Research (DZIF), Partner Site Gieβen-Marburg-Langen, Germany., Abu-Obaidah A; Ministry of Environment, Water and Agriculture (MEWA), Riyadh, Saudi Arabia., Alkarar A; Ministry of Environment, Water and Agriculture (MEWA), Riyadh, Saudi Arabia., Yoon IK; International Vaccine Institute, Seoul, South Korea., Azhar E; Department of Medical Laboratory Technology, Faculty of Applied Medical Sciences, King Abdulaziz University, Jeddah, Saudi Arabia.; Special Infectious Agents Unit, King Fahd Medical Research Center, King Abdulaziz University, Jeddah, Saudi Arabia., Lambe T; The Jenner Institute, University of Oxford, Oxford, OX3 7DQ, UK., Bayoumi F; Ministry of Environment, Water and Agriculture (MEWA), Riyadh, Saudi Arabia., Aldowerij A; Ministry of Environment, Water and Agriculture (MEWA), Riyadh, Saudi Arabia., Ibrahim OH; Ministry of Environment, Water and Agriculture (MEWA), Riyadh, Saudi Arabia., Gilbert SC; The Jenner Institute, University of Oxford, Oxford, OX3 7DQ, UK., Balkhy HH; Department of Infectious Disease Research, King Abdullah International Medical Research Center, Riyadh, Saudi Arabia.; King Saud bin Abdulaziz University for Health Sciences, Riyadh, Saudi Arabia.; Department of Infection Prevention and Control, Ministry of National Guard - Health Affairs, Riyadh, Saudi Arabia.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2019 Nov 08; Vol. 9 (1), pp. 16292. Date of Electronic Publication: 2019 Nov 08.
DOI: 10.1038/s41598-019-52730-4
Abstrakt: MERS-CoV seronegative and seropositive camels received a single intramuscular dose of ChAdOx1 MERS, a replication-deficient adenoviral vectored vaccine expressing MERS-CoV spike protein, with further groups receiving control vaccinations. Infectious camels with active naturally acquired MERS-CoV infection, were co-housed with the vaccinated camels at a ratio of 1:2 (infected:vaccinated); nasal discharge and virus titres were monitored for 14 days. Overall, the vaccination reduced virus shedding and nasal discharge (p = 0.0059 and p = 0.0274, respectively). Antibody responses in seropositive camels were enhancedby the vaccine; these camels had a higher average age than seronegative. Older seronegative camels responded more strongly to vaccination than younger animals; and neutralising antibodies were detected in nasal swabs. Further work is required to optimise vaccine regimens for younger seronegative camels.
Databáze: MEDLINE