Assessment of Time-Dependent Platelet Activation Using Extracellular Vesicles, CD62P Exposure, and Soluble Glycoprotein V Content of Platelet Concentrates with Two Different Platelet Additive Solutions.

Autor:	Valkonen S; EV Group, Molecular and Integrative Biosciences Research Program, Faculty of Biological and Environmental Sciences, University of Helsinki, Helsinki, Finland.; Finnish Red Cross Blood Service, Helsinki, Finland., Mallas B; Finnish Red Cross Blood Service, Helsinki, Finland., Impola U; Finnish Red Cross Blood Service, Helsinki, Finland., Valkeajärvi A; Finnish Red Cross Blood Service, Helsinki, Finland., Eronen J; Finnish Red Cross Blood Service, Helsinki, Finland., Javela K; Finnish Red Cross Blood Service, Helsinki, Finland., Siljander PR; EV Group, Molecular and Integrative Biosciences Research Program, Faculty of Biological and Environmental Sciences, University of Helsinki, Helsinki, Finland., Laitinen S; Finnish Red Cross Blood Service, Helsinki, Finland.
Jazyk:	angličtina
Zdroj:	Transfusion medicine and hemotherapy : offizielles Organ der Deutschen Gesellschaft fur Transfusionsmedizin und Immunhamatologie [Transfus Med Hemother] 2019 Aug; Vol. 46 (4), pp. 267-275. Date of Electronic Publication: 2019 May 07.
DOI:	10.1159/000499958
Abstrakt:	Novel analytical measures are needed to accurately monitor the properties of platelet concentrates (PCs). Since activated platelets produce platelet-derived extracellular vesicles (EVs), analyzing EVs of PCs may provide additional information about the condition of platelets. The prospect of using EVs as an auxiliary measure of platelet activation state was investigated by examining the effect of platelet additive solutions (PASs) on EV formation and platelet activation during PC storage. The time-dependent activation of platelets in PCs with PAS-B or with the further developed PAS-E was compared by measuring the exposure of CD62P by flow cytometry and the content of soluble glycoprotein V (sGPV) of PCs by an immunoassay. Changes in the concentration and size distribution of EVs were determined using nanoparticle tracking analysis. A time-dependent increase in platelet activation in PCs was demonstrated by increased CD62P ex-posure, sGPV content, and EV concentration. Using these strongly correlating parameters, PAS-B platelets were shown to be more activated compared to PAS-E platelets. Since the EV concentration correlated well with the established platelet activation markers CD62P and sGPV, it could potentially be used as a complementary parameter for platelet activation for PCs. More detailed characterization of the resulting EVs could help to understand how the PC components contribute the functional effects of transfused PCs. Competing Interests: The authors have no conflicts of interest to declare. (Copyright © 2019 by S. Karger AG, Basel.)
Databáze:	MEDLINE
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