Optimization and validation of automated dicentric chromosome analysis for radiological/nuclear triage applications.

Autor: Ryan TL; Radiation Emergency Assistance Center/Training Site, Cytogenetic Biodosimetry Laboratory, Oak Ridge Institute for Science and Education, Oak Ridge Associated Universities, Oak Ridge, TN 37830, USA., Escalona MB; Radiation Emergency Assistance Center/Training Site, Cytogenetic Biodosimetry Laboratory, Oak Ridge Institute for Science and Education, Oak Ridge Associated Universities, Oak Ridge, TN 37830, USA., Smith TL; Radiation Emergency Assistance Center/Training Site, Cytogenetic Biodosimetry Laboratory, Oak Ridge Institute for Science and Education, Oak Ridge Associated Universities, Oak Ridge, TN 37830, USA., Albanese J; Department of Therapeutic Radiology, Yale University School of Medicine, New Haven, CT, USA., Iddins CJ; Radiation Emergency Assistance Center/Training Site, Cytogenetic Biodosimetry Laboratory, Oak Ridge Institute for Science and Education, Oak Ridge Associated Universities, Oak Ridge, TN 37830, USA., Balajee AS; Radiation Emergency Assistance Center/Training Site, Cytogenetic Biodosimetry Laboratory, Oak Ridge Institute for Science and Education, Oak Ridge Associated Universities, Oak Ridge, TN 37830, USA. Electronic address: Adayabalam.balajee@orau.org.
Jazyk: angličtina
Zdroj: Mutation research. Genetic toxicology and environmental mutagenesis [Mutat Res Genet Toxicol Environ Mutagen] 2019 Nov; Vol. 847, pp. 503087. Date of Electronic Publication: 2019 Sep 23.
DOI: 10.1016/j.mrgentox.2019.503087
Abstrakt: Dicentric Chromosome Assay (DCA) is the most preferred cytogenetic technique for absorbed radiation dose assessment in exposed humans. However, DCA is somewhat impractical for triage application owing to its labor intensive and time consuming nature. Although lymphocyte culture for 48 h in vitro is inevitable for DCA, manual scoring of dicentric chromosomes (DCs) requires an additional time of 24-48 h, making the overall turnaround time of 72-96 h for dose estimation. To accelerate the speed of DC analysis for dose estimation, an automated tool was optimized and validated for triage mode of scoring. Several image training files were created to improve the specificity of automated DC analysis algorithm. Accuracy and efficiency of the automated (unsupervised) DC scoring was compared with the semi-automated scoring that involved human verification and correction of DCs (elimination of false positives and inclusion of true positives). DC scoring was performed by both automated and semi-automated modes for different doses of X-rays and γ-rays (0 Gy-5 Gy). Biodoses estimated from the frequencies of DCs detected by both automated (unsupervised) and semi-automated (supervised) scoring modes were grossly similar to the actual delivered doses in the range of 0.5 to 3 Gy of low LET radiation. We suggest that the automated DC tool can be effectively used for large scale radiological/nuclear incidents where a rapid segregation is essential for prioritizing moderately or severely exposed humans to receive appropriate medical countermeasures.
(Copyright © 2019 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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