| Autor: |
Uerlings J; Precision Livestock and Nutrition Unit, TERRA Teaching and Research Centre, Gembloux Agro-Bio Tech, ULiège, 5030 Gembloux, Belgium.; Research Foundation for Industry and Agriculture, National Scientific Research Foundation (FRIA-FNRS), 1000 Brussels, Belgium., Schroyen M; Precision Livestock and Nutrition Unit, TERRA Teaching and Research Centre, Gembloux Agro-Bio Tech, ULiège, 5030 Gembloux, Belgium., Bautil A; Laboratory of Food Chemistry and Biochemistry, Department of Microbial and Molecular Systems (M²S), KU Leuven, 3001 Leuven, Belgium., Courtin C; Laboratory of Food Chemistry and Biochemistry, Department of Microbial and Molecular Systems (M²S), KU Leuven, 3001 Leuven, Belgium., Richel A; Biomass and Green Technologies, TERRA Teaching and Research Center, Gembloux Agro-Bio Tech, ULiège, 5030 Gembloux, Belgium., Sureda EA; Precision Livestock and Nutrition Unit, TERRA Teaching and Research Centre, Gembloux Agro-Bio Tech, ULiège, 5030 Gembloux, Belgium., Bruggeman G; Royal Agrifirm Group, 7325 AW Apeldoorn, the Netherlands., Tanghe S; Royal Agrifirm Group, 7325 AW Apeldoorn, the Netherlands., Willems E; Royal Agrifirm Group, 7325 AW Apeldoorn, the Netherlands., Bindelle J; Precision Livestock and Nutrition Unit, TERRA Teaching and Research Centre, Gembloux Agro-Bio Tech, ULiège, 5030 Gembloux, Belgium., Everaert N; Precision Livestock and Nutrition Unit, TERRA Teaching and Research Centre, Gembloux Agro-Bio Tech, ULiège, 5030 Gembloux, Belgium. |
| Abstrakt: |
The inclusion of fibre-rich ingredients in diets is one possible strategy to enhance intestinal fermentation and positively impact gut ecology, barrier and immunity. Nowadays, inulin-type fructans are used as prebiotics in the feed of piglets to manipulate gut ecology for health purposes. Likewise, some by-products could be considered as sustainable and inexpensive ingredients to reduce gut disorders at weaning. In the present study, chicory root and pulp, citrus pulp, rye bran and soya hulls were tested in a three-step in vitro model of the piglet's gastro-intestinal tract combining a pepsin-pancreatin hydrolysis (digestion), a dialysis step using cellulose membranes (absorption) and a colonic batch fermentation (fermentation). The fermentation kinetics, SCFA and microbiota profiles in the fermentation broth were assessed as indicators of prebiotic activity and compared with the ones of inulin. The immunomodulatory effects of fermentation supernatant (FS) were investigated in cultured intestinal porcine epithelial cells (IPEC-J2) by high-throughput quantitative PCR. Chicory root displayed a rapid and extensive fermentation and induced the second highest butyrate ratio after inulin. Citrus pulp demonstrated high acetate ratios and induced elevated Clostridium clusters IV and XIVa levels. Chicory root and pulp FS promoted the intestinal barrier integrity with up-regulated tight and adherens junction gene expressions in comparison with inulin FS. Chicory pulp FS exerted anti-inflammatory effects in cultured IPEC-J2. The novel approach combining an in vitro fermentation model with IPEC-J2 cells highlighted that both chicory root and pulp appear to be promising ingredients and should be considered to promote intestinal health at weaning. |
