| Autor: |
de Souza VR; Laboratory of Functional Foods, Universidade Federal do Estado do Rio de Janeiro, Rio de Janeiro 22290-240, Brazil., Brum MCM; Cellular and Molecular Oncobiology Program, Research Centre, Instituto Nacional de Câncer, Rio de Janeiro 20231-050, Brazil., Guimarães IDS; Division of Clinical Research, Research Center, Instituto Nacional de Câncer, Rio de Janeiro 20231-050, Brazil., Dos Santos PF; Cellular and Molecular Oncobiology Program, Research Centre, Instituto Nacional de Câncer, Rio de Janeiro 20231-050, Brazil., do Amaral TO; Laboratory of Functional Foods, Universidade Federal do Estado do Rio de Janeiro, Rio de Janeiro 22290-240, Brazil., Abreu JP; Laboratory of Functional Foods, Universidade Federal do Estado do Rio de Janeiro, Rio de Janeiro 22290-240, Brazil., Passos T; Laboratory of Functional Foods, Universidade Federal do Estado do Rio de Janeiro, Rio de Janeiro 22290-240, Brazil., Freitas-Silva O; Embrapa Agroindústria de Alimentos, Empresa Brasileira de Pesquisa Agropecuária, Rio de Janeiro 23020-470, Brazil., Gimba ERP; Cellular and Molecular Oncobiology Program, Research Centre, Instituto Nacional de Câncer, Rio de Janeiro 20231-050, Brazil.; Department of Nature Sciences, Universidade Federal Fluminense, Rio das Ostras 28895-532, Brazil., Teodoro AJ; Laboratory of Functional Foods, Universidade Federal do Estado do Rio de Janeiro, Rio de Janeiro 22290-240, Brazil. |
| Abstrakt: |
Murici ( Byrsonima crassifolia (L.) Kunth and B. verbascifolia (L.) DC.) and tapereba ( Spondias mombin ) are Amazonian fruits that contain bioactive compounds. Biochemical and molecular characterization of these fruits can reveal their potential use in preventing diseases, including cancer. The extracts were characterized regarding the presence and profile of carotenoids by high-performance liquid chromatography (HPLC), total phenolic content by the Folin-Ciocalteu assay, and antioxidant activity by antioxidant value 2,2-diphenyl-1-picrylhydrazyl (DPPH) content analysis, 22,20-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) content analysis, Ferric-Reducing Ability of Plasma (FRAP), and Oxygen Radical Absorbance Capacity (ORAC) analysis. The extracts of tapereba and murici studied were important sources of total carotenoids and lutein, respectively. The extracts were then tested for their effect on the viability of the A2780 ovarian cancer (OC) cell line and its cisplatin (CDDP)-resistant derived cell line, called ACRP, by using MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assays. Their influence on cell cycle and apoptosis were analyzed by using flow cytometry. Murici and tapereba cell extracts exhibited a strong bioactivity by inhibiting A2780 and ACRP cell viability by 76.37% and 78.37%, respectively, besides modulating the cell cycle and inducing apoptotic cell death. Our results open new perspectives for the development of innovative therapeutic strategies using these Amazon fruit extracts to sensitize ovarian cancer cells to current chemotherapeutic options. |
