A highly specific and sensitive nanoimmunosensor for the diagnosis of neuromyelitis optica spectrum disorders.

Autor: de Souza Moraes A; Institute of Tropical Medicine of São Paulo, University of São Paulo, São Paulo, São Paulo, 05403000, Brazil.; Department of Physics, Chemistry and Mathematics, Federal University of São Carlos, Sorocaba, São Paulo, 18052780, Brazil.; Nanoneurobiophysics research group (GNN), Federal University of São Carlos, Sorocaba, São Paulo, 18052780, Brazil., Brum DG; Nanoneurobiophysics research group (GNN), Federal University of São Carlos, Sorocaba, São Paulo, 18052780, Brazil.; Department of Neurology, Psychology and Psychiatry, São Paulo State University, Botucatu, São Paulo, 18618687, Brazil., Ierich JCM; Institute of Tropical Medicine of São Paulo, University of São Paulo, São Paulo, São Paulo, 05403000, Brazil.; Department of Physics, Chemistry and Mathematics, Federal University of São Carlos, Sorocaba, São Paulo, 18052780, Brazil.; Nanoneurobiophysics research group (GNN), Federal University of São Carlos, Sorocaba, São Paulo, 18052780, Brazil., Higa AM; Institute of Tropical Medicine of São Paulo, University of São Paulo, São Paulo, São Paulo, 05403000, Brazil.; Department of Physics, Chemistry and Mathematics, Federal University of São Carlos, Sorocaba, São Paulo, 18052780, Brazil.; Nanoneurobiophysics research group (GNN), Federal University of São Carlos, Sorocaba, São Paulo, 18052780, Brazil., Assis ASJ; Department of Physics, Chemistry and Mathematics, Federal University of São Carlos, Sorocaba, São Paulo, 18052780, Brazil.; Nanoneurobiophysics research group (GNN), Federal University of São Carlos, Sorocaba, São Paulo, 18052780, Brazil., Miyazaki CM; Department of Physics, Chemistry and Mathematics, Federal University of São Carlos, Sorocaba, São Paulo, 18052780, Brazil., Shimizu FM; São Carlos Institute of Physics, University of São Paulo, São Carlos, São Paulo, 13560970, Brazil., Peroni LA; Rheabiotech Laboratory of Research and Development, Campinas, São Paulo, 13084791, Brazil., Machini MT; Department of Biochemistry, Institute of Chemistry, University of São Paulo, São Paulo, 05508000, Brazil., Barreira AA; Department of Neurosciences and Behavioural Sciences, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, São Paulo, Brazil., Ferreira M; Department of Physics, Chemistry and Mathematics, Federal University of São Carlos, Sorocaba, São Paulo, 18052780, Brazil., Oliveira ON Jr; São Carlos Institute of Physics, University of São Paulo, São Carlos, São Paulo, 13560970, Brazil., Leite FL; Department of Physics, Chemistry and Mathematics, Federal University of São Carlos, Sorocaba, São Paulo, 18052780, Brazil. fabioleite@ufscar.br.; Nanoneurobiophysics research group (GNN), Federal University of São Carlos, Sorocaba, São Paulo, 18052780, Brazil. fabioleite@ufscar.br.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2019 Nov 06; Vol. 9 (1), pp. 16136. Date of Electronic Publication: 2019 Nov 06.
DOI: 10.1038/s41598-019-52506-w
Abstrakt: A precise diagnosis for neuromyelitis optica spectrum disorders (NMOSD) is crucial to improve patients' prognostic, which requires highly specific and sensitive tests. The cell-based assay with a sensitivity of 76% and specificity of 100% is the most recommended test to detect anti-aquaporin-4 antibodies (AQP4-Ab). Here, we tested four AQP4 external loop peptides (AQP4 61-70 , AQP4 131-140 , AQP4 141-150 , and AQP4 201-210 ) with an atomic force microscopy nanoimmunosensor to develop a diagnostic assay. We obtained the highest reactivity with AQP4 61-70 -nanoimunosensor. This assay was effective in detecting AQP4-Ab in sera of NMOSD patients with 100% specificity (95% CI 63.06-100), determined by the cut-off adhesion force value of 241.3 pN. NMOSD patients were successfully discriminated from a set of healthy volunteers, patients with multiple sclerosis, and AQP4-Ab-negative patients. AQP4 61-70 sensitivity was 81.25% (95% CI 56.50-99.43), slightly higher than with the CBA method. The results with the AQP4 61-70 -nanoimmunosensor indicate that the differences between NMOSD seropositive and seronegative phenotypes are related to disease-specific epitopes. The absence of AQP4-Ab in sera of NMOSD AQP4-Ab-negative patients may be interpreted by assuming the existence of another potential AQP4 peptide sequence or non-AQP4 antigens as the antibody target.
Databáze: MEDLINE
Nepřihlášeným uživatelům se plný text nezobrazuje