| Autor: |
Reale E; Department of Physics & INFN, University of Torino, 10125, Torino, Italy., Taverna D; Molecular Biotechnology Center (MBC), 10126, Torino, Italy.; Department of Molecular Biotechnology & Health Sciences, 10126, Torino, Italy.; Center for Complex Systems in Molecular Biology & Medicine, University of Torino, 10123, Torino, Italy., Cantini L; Institut Curie, PSL Research University, INSERM U900, Paris, France.; Computational Systems Biology Team, Institut de Biologie de l'Ecole Normale Supérieure, CNRS UMR8197, INSERM U1024, Ecole Normale Supérieure, Paris Sciences et Lettres Research University, 75005 Paris, France., Martignetti L; Institut Curie, PSL Research University, INSERM U900, Paris, France., Osella M; Department of Physics & INFN, University of Torino, 10125, Torino, Italy., De Pittà C; Department of Biology, University of Padova, 3522, Padova, Italy., Virga F; Molecular Biotechnology Center (MBC), 10126, Torino, Italy.; Department of Molecular Biotechnology & Health Sciences, 10126, Torino, Italy., Orso F; Molecular Biotechnology Center (MBC), 10126, Torino, Italy.; Department of Molecular Biotechnology & Health Sciences, 10126, Torino, Italy.; Center for Complex Systems in Molecular Biology & Medicine, University of Torino, 10123, Torino, Italy., Caselle M; Department of Physics & INFN, University of Torino, 10125, Torino, Italy. |
| Abstrakt: |
Aim: Growing evidence shows a strong interplay between post-transcriptional regulation, mediated by miRNAs (miRs) and epigenetic regulation. Nevertheless, the number of experimentally validated miRs (called epi-miRs) involved in these regulatory circuitries is still very small. Material & methods: We propose a pipeline to prioritize candidate epi-miRs and to identify potential epigenetic interactors of any given miR starting from miR transfection experiment datasets. Results & conclusion: We identified 34 candidate epi-miRs: 19 of them are known epi-miRs, while 15 are new. Moreover, using an in-house generated gene expression dataset, we experimentally proved that a component of the polycomb-repressive complex 2, the histone methyltransferase enhancer of zeste homolog 2 (EZH2), interacts with miR-214, a well-known prometastatic miR in melanoma and breast cancer, highlighting a miR-214-EZH2 regulatory axis potentially relevant in tumor progression. |
