| Autor: |
van Hout D; Julius Center for Health Sciences and Primary Care, University Medical Center Utrecht, University Utrecht, Huispost nr. STR 6.131, P.O. Box 85500, 3508 GA, Utrecht, the Netherlands. D.vanHout-3@umcutrecht.nl., Janssen AB; Department of Medical Microbiology, University Medical Center Utrecht, University Utrecht, Utrecht, the Netherlands., Rentenaar RJ; Department of Medical Microbiology, University Medical Center Utrecht, University Utrecht, Utrecht, the Netherlands., Vlooswijk JPM; Department of Medical Microbiology, University Medical Center Utrecht, University Utrecht, Utrecht, the Netherlands., Boel CHE; Department of Medical Microbiology, University Medical Center Utrecht, University Utrecht, Utrecht, the Netherlands., Bonten MJM; Julius Center for Health Sciences and Primary Care, University Medical Center Utrecht, University Utrecht, Huispost nr. STR 6.131, P.O. Box 85500, 3508 GA, Utrecht, the Netherlands.; Department of Medical Microbiology, University Medical Center Utrecht, University Utrecht, Utrecht, the Netherlands. |
| Abstrakt: |
The objective of this study was to determine the value of using SuperPolymyxin™ selective medium (ELITech Group, Puteaux, France) in addition to conventional non-selective inoculation methods in the detection of acquired colistin resistance in a Dutch intensive care unit (ICU) that routinely uses selective decontamination of the digestive tract (SDD). We performed a cross-sectional study with prospective data collection in a tertiary-care ICU. All consecutive surveillance rectal swabs of ICU-patients receiving SDD were included and cultured in an observer-blinded approach using (1) a conventional culture method using non-selective media and (2) SuperPolymyxin™ selective medium. MIC values for colistin of non-intrinsically colistin-resistant Gram-negative isolates were determined with broth microdilution (BMD) using Sensititre™ and colistin resistance was confirmed using BMD according to EUCAST guidelines. One thousand one hundred five rectal swabs of 428 unique ICU-patients were inoculated using both culture methods, yielding 346 and 84 Gram-negative isolates for BMD testing with the conventional method and SuperPolymyxin™ medium, of which 308 and 80 underwent BMD, respectively. The number of identified rectal carriers of isolates with acquired colistin resistance was 3 (0.7%) for the conventional method, 4 (0.9%) for SuperPolymyxin™, and 5 (1.2%) for both methods combined. The number of isolates with acquired colistin resistance was 4 (1.0%) for the conventional method, 8 (2.1%) for SuperPolymyxin™ and 9 (2.3%) for both methods combined. In a surveillance setting of low prevalence of acquired colistin resistance in patients that receive SDD in a Dutch tertiary-care ICU, SuperPolymyxin™ had a higher diagnostic yield than conventional inoculation methods, but the combination of both had the highest diagnostic yield. |
Full text from SpringerLink