Effects of Pyrene on Human Liver HepG2 Cells: Cytotoxicity, Oxidative Stress, and Transcriptomic Changes in Xenobiotic Metabolizing Enzymes and Inflammatory Markers with Protection Trial Using Lycopene.

Autor: Ma JK; School of Food & Pharmaceutical Engineering, Zhaoqing University, Zhaoqing 526061, China., Saad Eldin WF; Educational Veterinary Hospital, Faculty of Veterinary Medicine, Zagazig University, Zagazig 44519, Egypt., El-Ghareeb WR; Department of Veterinary Public Health and Animal Husbandry, College of Veterinary Medicine, King Faisal University, Al Hofuf, Saudi Arabia., Elhelaly AE; Department of Food Hygiene and Control, Faculty of Veterinary Medicine, Suez Canal University, Ismailia 41522, Egypt.; Center for Emerging Infectious Diseases, School of Medicine, Gifu University, Gifu 501-1193, Japan., Khedr MHE; Department of Veterinary Hygiene, Faculty of Veterinary Medicine, Zagazig University, Zagazig 44519, Egypt., Li X; College of Environmental and Chemical Engineering, Zhaoqing University, Zhaoqing 526061, China., Huang XC; School of Food & Pharmaceutical Engineering, Zhaoqing University, Zhaoqing 526061, China.
Jazyk: angličtina
Zdroj: BioMed research international [Biomed Res Int] 2019 Oct 09; Vol. 2019, pp. 7604851. Date of Electronic Publication: 2019 Oct 09 (Print Publication: 2019).
DOI: 10.1155/2019/7604851
Abstrakt: Pyrene is one of the major polycyclic aromatic hydrocarbons formed during heat treatment of meat and in car exhausts; however, few studies have investigated pyrene-induced adverse effects on human cell lines. This study aimed at the investigation of pyrene-induced cytotoxicity and oxidative damage in human liver HepG2 cells at environmentally relevant concentrations. Pyrene-induced changes in mRNA expression of xenobiotic metabolizing enzymes (XMEs), xenobiotic transporters, antioxidant enzymes, and inflammatory markers were investigated using real-time PCR. As a protection trial, the ameliorative effects of lycopene, a carotenoid abundantly found in tomato, were investigated. The possible mechanisms behind such effects were examined via studying the co exposure effects of pyrene and lycopene on regulatory elements including the aryl hydrocarbon receptor (Air) and elytroid 2-related factor 2 (RF). The achieved results indicated that pyrene caused significant cytotoxicity at 50 n, with a clear production of reactive oxygen species (ROS) in a dose-dependent manner. Pyrene upregulated mRNA expression of phase I enzymes including CYP1A1, 1A2, and CYP1B1 and inflammatory markers including TNF α and Cox2. However, pyrene significantly downregulated phase II enzymes, xenobiotic transporters, and antioxidant enzymes. Interestingly, lycopene significantly reduced pyrene-induced cytotoxicity and ROS production. Moreover, lycopene upregulated detoxification and antioxidant enzymes, probably via its regulatory effects on Air- and RF-dependent pathways.
Competing Interests: The authors declare that they have no conflicts of interest.
(Copyright © 2019 Jin-Kui Ma et al.)
Databáze: MEDLINE
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