Biological depletion of neutrophils attenuates pro-inflammatory markers and the development of the psoriatic phenotype in a murine model of psoriasis.

Autor: Han G; Department of Dermatology, Icahn School of Medicine at Mount Sinai, New York, NY, United States of America. Electronic address: george.han@mountsinai.org., Havnaer A; Department of Dermatology, Icahn School of Medicine at Mount Sinai, New York, NY, United States of America; The Warren Alpert Medical School of Brown University, Providence, RI, United States of America., Lee HH; Department of Biomedical Sciences, NYIT College of Osteopathic Medicine, New York Institute of Technology, Old Westbury, NY, United States of America., Carmichael DJ; Department of Biomedical Sciences, NYIT College of Osteopathic Medicine, New York Institute of Technology, Old Westbury, NY, United States of America., Martinez LR; Department of Biomedical Sciences, NYIT College of Osteopathic Medicine, New York Institute of Technology, Old Westbury, NY, United States of America; Department of Biological Sciences, The Border Biomedical Research Center, The University of Texas at El Paso, TX, United States of America.
Jazyk: angličtina
Zdroj: Clinical immunology (Orlando, Fla.) [Clin Immunol] 2020 Jan; Vol. 210, pp. 108294. Date of Electronic Publication: 2019 Oct 31.
DOI: 10.1016/j.clim.2019.108294
Abstrakt: Although neutrophils are considered a histologic hallmark of psoriasis, their pathophysiologic role in psoriasis remains unclear. We characterized the effects of neutrophil depletion via injection of monoclonal antibody 1A8 on the development of imiquimod (IMQ)-induced psoriatic lesions in a murine model. Lesions were followed with photographs and histologic analysis, revealing reduced psoriasiform scale and epidermal hyperplasia in neutrophil-depleted. ELISA and flow cytometry were used to determine relative levels of cytokines and immune cells. Compared to controls, IMQ-treated neutropenic mice had significantly lower levels of macrophages in tissue samples (P < .05) and displayed significantly lower numbers of CD4 + T-cells (P < .05). Neutropenic animals exhibited lower levels of TNF-α, IFN-γ, and IL-1β than controls (P < .05). These results show that neutropenia reduces the development of psoriasiform skin lesions and substantially decreases infiltration of pro-inflammatory cytokines and immune cells to IMQ-induced cutaneous lesions, suggesting an active role of neutrophils in maintaining inflammation in psoriasis.
(Copyright © 2019 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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