Sensitive Determination of C-Alkylated Flavonoids by HPLC-ESI-MS/MS Using Multiple Reaction Monitoring Approach: Pseudarthria hookeri as a Case Study.

Autor: Faraz I; HEJ Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi, University Road, Karachi-75270, Sindh, Pakistan., Ali A; HEJ Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi, University Road, Karachi-75270, Sindh, Pakistan., Ul Haq F; HEJ Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi, University Road, Karachi-75270, Sindh, Pakistan., Tchamgoue J; Department of Chemistry, Higher Teachers Training College, University of Yaoundé I, P.O. Box 47, Yaoundé, Cameroon., Kouam SF; Department of Chemistry, Higher Teachers Training College, University of Yaoundé I, P.O. Box 47, Yaoundé, Cameroon., Miana GA; Riphah Institute of Pharmaceutical Sciences, Riphah International University, Islamabad-44000, Pakistan, and., Musharraf SG; HEJ Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi, University Road, Karachi-75270, Sindh, Pakistan.; Dr. Panjwani Centre for Molecular Medicine and Drug Research, International Center for Chemical and Biological Sciences, University of Karachi, Karachi 75270, Pakistan.
Jazyk: angličtina
Zdroj: Journal of chromatographic science [J Chromatogr Sci] 2020 Jan 17; Vol. 57 (10), pp. 944-949.
DOI: 10.1093/chromsci/bmz072
Abstrakt: One of the major problems with the formulation of herbal medicines is the quality control of plant material to ensure its efficacy and safety. Quality control of medicinal plants requires analysis of many bioactive compounds present in the plant. C-alkylated flavonoids are an important bioactive subclass of flavonoids. A simple, rapid, sensitive and selective method is presented here for the quantification of bioactive C-alkylated flavonoids. This is the first quantitative method for analysis of C-alkylated flavonoids based on the multiple reaction monitoring (MRM) approach so far. This study focuses on method development for quantification of bioactive C-alkylated flavonoids. Quantification of a total of five C-alkylated flavonoids was done employing the MRM approach on an HPLC-QqQ-MS instrument. LODs and LOQs for quantified flavonoids were in the range of 0.41-1.32 and 1.23-3.96 ng/mL, respectively. Linear calibration curves between 25 and 1500 ng/mL were obtained with the regression coefficients of ≥0.996. Accuracy (% bias) and precision (% RSD) of the analyses were found to be less than 5%. Developed HPLC-ESI-MS/MS can be employed as a quality control method of plant raw materials.
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Databáze: MEDLINE
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