GABA synaptopathy promotes the elevation of caspases 3 and 9 as pro-apoptotic markers in Egyptian patients with autism spectrum disorder.

Autor: El-Ansary A; Central Laboratory, Female Centre for Scientific and Medical Studies, King Saud University, Riyadh, Saudi Arabia.; Therapeutic Chemistry Department, National Research Centre, Dokki, Cairo, Egypt.; CONEM Saudi Autism Research Group, King Saud University, Riyadh, Saudi Arabia.; Department of Physiology, College of Medicine, King Saud University, Riyadh, Saudi Arabia.; Autism Research and Treatment Center, Riyadh, Saudi Arabia., Zayed N; Therapeutic Chemistry Department, National Research Centre, Dokki, Cairo, Egypt., Al-Ayadhi L; Department of Physiology, College of Medicine, King Saud University, Riyadh, Saudi Arabia.; Autism Research and Treatment Center, Riyadh, Saudi Arabia., Qasem H; Autism Research and Treatment Center, Riyadh, Saudi Arabia., Anwar M; Department of Research on Children with Special Needs, National Research Centre, Giza, Egypt., Meguid NA; Department of Research on Children with Special Needs, National Research Centre, Giza, Egypt.; CONEM Egypt Child Brain Research Group, National Research Center, Giza, Egypt., Bhat RS; Biochemistry Department, College of Sciences, King Saud University, Riyadh, Saudi Arabia., Doşa MD; Department of Pharmacology, Faculty of Medicine, Ovidius University, Constanța, Romania. monicadanielad@yahoo.com., Chirumbolo S; Department of Neuroscience, Biomedicine and Movement Sciences, University of Verona, Verona, Italy.; CONEM Scientific Secretary, Verona, Italy., Bjørklund G; Council for Nutritional and Environmental Medicine (CONEM), Toften 24, 8610, Mo i Rana, Norway. bjorklund@conem.org.
Jazyk: angličtina
Zdroj: Acta neurologica Belgica [Acta Neurol Belg] 2021 Apr; Vol. 121 (2), pp. 489-501. Date of Electronic Publication: 2019 Oct 31.
DOI: 10.1007/s13760-019-01226-z
Abstrakt: Autism spectrum disorder (ASD) is classified as a neurodevelopmental disorder characterized by reduced social communication as well as repetitive behaviors. Many studies have proved that defective synapses in ASD influence how neurons in the brain connect and communicate with each other. Synaptopathies arise from alterations that affecting the integrity and/or functionality of synapses and can contribute to synaptic pathologies. This study investigated the GABA levels in plasma being an inhibitory neurotransmitter, caspase 3 and 9 as pro-apoptotic proteins in 20 ASD children and 20 neurotypical controls using the ELISA technique. Analysis of receiver-operating characteristic (ROC) of the data that was obtained to evaluate the diagnostic value of the aforementioned evaluated biomarkers. Pearson's correlations and multiple regressions between the measured variables were also done. While GABA level was reduced in ASD patients, levels of caspases 3 and 9 were significantly higher when compared to neurotypical control participants. ROC and predictiveness curves showed that caspases 3, caspases 9, and GABA might be utilized as predictive markers in autism diagnosis. The present study indicates that the presence of GABAergic dysfunction promotes apoptosis in Egyptian ASD children. The obtained GABA synaptopathies and their connection with apoptosis can both relate to neuronal excitation, and imbalance of the inhibition system, which can be used as reliable predictive biomarkers for ASD.
Databáze: MEDLINE
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