Chemical variability of Rhododendron tomentosum (Ledum palustre) essential oils and their pro-apoptotic effect on lymphocytes and rheumatoid arthritis synoviocytes.

Autor: Jesionek A; Department of Pharmacognosy, Medical University of Gdansk, Hallera 107, 80-416 Gdansk, Poland., Kokotkiewicz A; Department of Pharmacognosy, Medical University of Gdansk, Hallera 107, 80-416 Gdansk, Poland., Mikosik-Roczynska A; Department of Pathophysiology, Medical University of Gdansk, Debinki 7, 80-211 Gdansk, Poland., Ciesielska-Figlon K; Department of Pathophysiology, Medical University of Gdansk, Debinki 7, 80-211 Gdansk, Poland., Luczkiewicz P; II Clinic of Orthopaedics and Kinetic Organ Traumatology, Medical University of Gdansk, Smoluchowskiego 17, 80-214 Gdansk, Poland., Bucinski A; Department of Biopharmacy, Ludwik Rydygier Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Torun, Jurasza 2, 85-089 Bydgoszcz, Poland., Daca A; Department of Pathology and Experimental Rheumatology, Medical University of Gdansk, Debinki 7, 80-211 Gdansk, Poland., Witkowski JM; Department of Pathophysiology, Medical University of Gdansk, Debinki 7, 80-211 Gdansk, Poland., Bryl E; Department of Pathology and Experimental Rheumatology, Medical University of Gdansk, Debinki 7, 80-211 Gdansk, Poland., Zabiegala B; Department of Analytical Chemistry, Gdansk University of Technology, G. Narutowicza 11/12, 80-233 Gdansk, Poland., Luczkiewicz M; Department of Pharmacognosy, Medical University of Gdansk, Hallera 107, 80-416 Gdansk, Poland. Electronic address: mlucz@gumed.edu.pl.
Jazyk: angličtina
Zdroj: Fitoterapia [Fitoterapia] 2019 Nov; Vol. 139, pp. 104402. Date of Electronic Publication: 2019 Oct 28.
DOI: 10.1016/j.fitote.2019.104402
Abstrakt: Rhododendron tomentosum (Ledum palustre) is an aromatic plant traditionally used for alleviating rheumatic complaints which makes it a potential candidate for a natural drug in rheumatoid arthritis (RA) treatment. However, the effects of plants' volatiles on apoptosis of synovial fibroblasts and infiltrating leucocytes of RA synovia, have not been reported. Volatile fraction of R. tomentosum is chemically variable and chemotypes of the plants need to be defined if the oil is to be used for therapeutic purposes. In the presented work, cluster analysis of literature data enabled to define 10 chemotypes of the plant. The volatile fractions of known composition were then tested for bioactivity using a RA-specific in vitro models. Essential oils of two wild types (γ-terpineol and palustrol/ledol type) and one in vitro chemotype (ledene oxide type) were obtained by hydrodistillation and their bioactivity was tested in two in vitro models: I - peripheral blood lymphocytes of healthy volunteers and II - synoviocytes and immune cells isolated from synovia of RA patients. The influence of oils on blood lymphocytes' proliferation and apoptosis rates of synovia-derived cells was determined by flow cytometry. Dose-dependent inhibitory effect of the serial dilutions of R. tomentosum oils on proliferation rates of blood lymphocytes was found. At 1:400 dilutions, all the tested oils increased the number of necrotic cells in synovial fibroblasts from RA synovia. Additionally, increased proportions of late apoptotic cells were observed in leucocyte populations subjected to oils at 1:400 dilution.
(Copyright © 2019 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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