Fusarium graminearum FgCWM1 Encodes a Cell Wall Mannoprotein Conferring Sensitivity to Salicylic Acid and Virulence to Wheat.

Autor: Zhang YZ; State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China Sichuan Agricultural University, Chengdu 611130, Sichuan, China. zhangyazhou@stu.sicau.edu.cn.; Triticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, Sichuan, China. zhangyazhou@stu.sicau.edu.cn., Chen Q; Triticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, Sichuan, China. qingchen83@sicau.edu.cn., Liu CH; Triticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, Sichuan, China. rainbow@stu.sicau.edu.cn., Lei L; Triticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, Sichuan, China. leilu@stu.sicau.edu.cn., Li Y; Triticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, Sichuan, China. liyang1@stu.sicau.edu.cn., Zhao K; Triticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, Sichuan, China. zhaokan6137@163.com., Wei MQ; Triticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, Sichuan, China. 2018212010@stu.sicau.edu.cn., Guo ZR; Triticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, Sichuan, China. guozhenru@stu.sicau.edu.cn., Wang Y; Triticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, Sichuan, China. wangyan6@stu.sicau.edu.cn., Xu BJ; Triticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, Sichuan, China. binjiexu@outlook.com., Jiang YF; Triticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, Sichuan, China. jiangyunfeng2018@163.com., Kong L; Triticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, Sichuan, China. kongli@sicau.edu.cn., Liu YL; Triticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, Sichuan, China. liuyanlin@sicau.edu.cn., Lan XJ; Triticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, Sichuan, China. lanxiujin@163.com., Jiang QT; State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China Sichuan Agricultural University, Chengdu 611130, Sichuan, China. qiantaojiang@sicau.edu.cn.; Triticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, Sichuan, China. qiantaojiang@sicau.edu.cn., Ma J; Triticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, Sichuan, China. jianma@sicau.edu.cn., Wang JR; State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China Sichuan Agricultural University, Chengdu 611130, Sichuan, China. jirui.wang@gmail.com.; Triticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, Sichuan, China. jirui.wang@gmail.com., Chen GY; State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China Sichuan Agricultural University, Chengdu 611130, Sichuan, China. guoyuech74@hotmail.com.; Triticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, Sichuan, China. guoyuech74@hotmail.com., Wei YM; State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China Sichuan Agricultural University, Chengdu 611130, Sichuan, China. ymwei@sicau.edu.cn.; Triticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, Sichuan, China. ymwei@sicau.edu.cn., Zheng YL; State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China Sichuan Agricultural University, Chengdu 611130, Sichuan, China. ylzheng@sicau.edu.cn.; Triticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, Sichuan, China. ylzheng@sicau.edu.cn., Qi PF; Triticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, Sichuan, China. pengfeiqi@hotmail.com.
Jazyk: angličtina
Zdroj: Toxins [Toxins (Basel)] 2019 Oct 29; Vol. 11 (11). Date of Electronic Publication: 2019 Oct 29.
DOI: 10.3390/toxins11110628
Abstrakt: Fusarium graminearum causes Fusarium head blight (FHB), a devastating disease of wheat. Salicylic acid (SA) is involved in the resistance of wheat to F. graminearum . Cell wall mannoprotein (CWM) is known to trigger defense responses in plants, but its role in the pathogenicity of F. graminearum remains unclear. Here, we characterized FgCWM1 ( FG05_11315 ), encoding a CWM in F. graminearum . FgCWM1 was highly expressed in wheat spikes by 24 h after initial inoculation and was upregulated by SA. Disruption of FgCWM1 (Δ FgCWM1 ) reduced mannose and protein accumulation in the fungal cell wall, especially under SA treatment, and resulted in defective fungal cell walls, leading to increased fungal sensitivity to SA. The positive role of FgCWM1 in mannose and protein accumulation was confirmed by its expression in Saccharomyces cerevisiae . Compared with wild type (WT), Δ FgCWM1 exhibited reduced pathogenicity toward wheat, but it produced the same amount of deoxynivalenol both in culture and in spikes. Complementation of Δ FgCWM1 with FgCWM1 restored the WT phenotype. Localization analyses revealed that FgCWM1 was distributed on the cell wall, consistent with its structural role. Thus, FgCWM1 encodes a CWM protein that plays an important role in the cell wall integrity and pathogenicity of F. graminearum .
Databáze: MEDLINE
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