Seronegative hepatitis C virus infection in Polish blood donors-Virological characteristics of index donations and follow-up observations.

Autor: Grabarczyk P; Department of Virology, Institute of Haematology and Transfusion Medicine, Warsaw, Poland., Kubicka-Russel D; Department of Virology, Institute of Haematology and Transfusion Medicine, Warsaw, Poland., Kopacz A; Department of Virology, Institute of Haematology and Transfusion Medicine, Warsaw, Poland., Liszewski G; Department of Virology, Institute of Haematology and Transfusion Medicine, Warsaw, Poland., Sulkowska E; Department of Virology, Institute of Haematology and Transfusion Medicine, Warsaw, Poland., Zwolińska P; Department of Virology, Institute of Haematology and Transfusion Medicine, Warsaw, Poland., Madaliński K; Department of Virology, National Institute of Public Health-National Institute of Hygiene, Warsaw, Poland., Marek M; Labolatory of Infectious Diseases Transmitted by Blood, Regional Blood Transfusion Center, Kalisz, Poland., Szabelewska M; Department of Testing for Infectious Diseases Transmitted by Transfusion, Military Blood Transfusion Center, Warsaw, Poland., Świątek E; Laboratory of Infectious Diseases Serodiagnostics, Regional Blood Transfusion Center, Wrocław, Poland., Laskus T; Department of Adult Infectious Diseases, Warsaw Medical University, Warsaw, Poland., Radkowski M; Department of Immunopathology of Infectious and Parasitic Diseases, Warsaw Medical University, Warsaw, Poland.
Jazyk: angličtina
Zdroj: Journal of medical virology [J Med Virol] 2020 Mar; Vol. 92 (3), pp. 339-347. Date of Electronic Publication: 2019 Nov 21.
DOI: 10.1002/jmv.25617
Abstrakt: Nucleic acid testing (NAT) was implemented in Poland in 1999 for screening of plasma for fractionation and for all blood donors in 2002. To analyze seronegative NAT-positive samples representing hepatitis C virus (HCV) window-period (WP) in the years 2000 to 2016 and to determine infection outcome. We analyzed results of 17 502 739 donations screened in minipools (6-48) or individually. Index samples underwent viral load (VL) quantification, genotyping and Ag, and anti-HCV re-testing using chemiluminescence (CMIA), electrochemiluminescence (ECLIA), and fourth-generation enzyme-linked immunosorbent assay (IV EIA) assays. HCV-seronegative infections were identified in 126 donations (7.2/mln donations; 95% confidential intervals, 6.0-8.6). Frequency of NAT yields was decreasing over time. Of the initial 126 seronegative index cases 106 were retested: 32.1% were reactive in IV EIA, 11.3% in ECLIA, and 1.9% in CMIA. The lowest VL correlated with absent anti-HCV and HCV Ag, while VL was highest when the antigen was detectable and then it decreased when anti-HCV appeared at a level detectable by sensitive third generation tests while retesting. The proportion of genotype 1 was 38.9% in samples positive only for HCV RNA and 71.4% in samples that were anti-HCV reactive in re-testing. In parallel, genotype 3 frequency was 50% in the former group and 21% in the latter. NAT is an effective measure to limit HCV transmission by transfusion and IV EIA seems to have higher clinical sensitivity than ECLIA. Samples representing likely successive phases of early HCV infection were characterized by different genotype distribution probably due to very early elimination of genotype 3.
(© 2019 The Authors. Journal of Medical Virology published by Wiley Periodicals, Inc.)
Databáze: MEDLINE
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