Molecular and morphometric identification of Trypanosoma (Megatrypanum) minasense in blood samples of marmosets (Callithrix: Callithrichidae) from the city of Rio de Janeiro, Brazil.

Autor: Coimbra DP; Programa de Pós-graduação em Biologia Animal (PPGBA), Instituto de Ciências Biológicas e da Saúde (ICBS), Universidade Federal Rural do Rio de Janeiro (UFRRJ), Seropédica, RJ 23897-000, Brazil. Electronic address: coimbradp@gmail.com., Penedo DM; Programa de Pós-graduação em Biologia Animal (PPGBA), Instituto de Ciências Biológicas e da Saúde (ICBS), Universidade Federal Rural do Rio de Janeiro (UFRRJ), Seropédica, RJ 23897-000, Brazil., Silva MOM; Programa de Pós-graduação em Biologia Animal (PPGBA), Instituto de Ciências Biológicas e da Saúde (ICBS), Universidade Federal Rural do Rio de Janeiro (UFRRJ), Seropédica, RJ 23897-000, Brazil., Abreu APM; Programa de Pós-graduação em Ciências Veterinárias, UFRRJ, Seropédica, RJ 23897-000, Brazil., Silva CB; Departamento de Parasitologia Animal, Instituto de Veterinária, UFRRJ, Seropédica, RJ 23897-000, Brazil., Verona CE; Fundação RioZoo, Rio de Janeiro, RJ 20940-040, Brazil., Heliodoro GC; Jardim Botânico do Rio de Janeiro, Rio de Janeiro, RJ 22460-030, Brazil., Massard CL; Departamento de Parasitologia Animal, Instituto de Veterinária, UFRRJ, Seropédica, RJ 23897-000, Brazil., Nogueira DM; Departamento de Genética, ICBS, UFRRJ, Seropédica, RJ 23897-000, Brazil.
Jazyk: angličtina
Zdroj: Parasitology international [Parasitol Int] 2020 Apr; Vol. 75, pp. 101999. Date of Electronic Publication: 2019 Oct 24.
DOI: 10.1016/j.parint.2019.101999
Abstrakt: Callithrix jacchus and C. penicillata marmosets are invasive to the state of Rio de Janeiro, Brazil, threatening the native and vulnerable C. aurita. Both invasive species can be hosts of Trypanosoma cruzi, T. minasense, T. rangeli and T. devei. We aim to investigate the occurrence of trypanosomatids in Callithrix sp. from Jardim Botânico do Rio de Janeiro, located in a central and populous area of the city. Fifteen marmosets were captured. Blood samples were collected for light microscopy and molecular genetics analysis. Parasites morphometric values were evaluated for species identification. DNA was extracted from blood samples by phenol-chloroform method, for partial amplification of the 18S rRNA gene. PCR products were sequenced and aligned using BLAST®. A maximum likelihood phylogenetic tree was constructed to analyze the proximity between the observed sequences. By light microscopy, trypomastigotes were detected in five of the fifteen marmosets. Morphometric measurements and size polymorphism corresponded to those previously described for T. minasense. The DNA sequences of approximately 600 base pairs of the 18S rRNA gene were obtained for three samples with 99% identity with T. minasense sequence, forming a cluster in the phylogenetic tree and corroborating morphometric analysis. Trypanosoma minasense is a highly specific parasite to non-human primates considered as non-pathogenic. There is no evidence of infection in humans and these parasite findings from invasive marmosets do not support additional risks for the native species.
(Copyright © 2019 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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