Differential Expression of Monocarboxylate Transporter 1 and Ancillary Protein CD147 in Red Blood Cells of Show Jumping Horses.

Autor: Júnior WHF; Departamento de Morfologia e Fisiologia Animal, Faculdades de Ciências Agrárias e Veterinárias, UNESP Univ Estadual Paulista, Campus de Jaboticabal, Laboratório de Farmacologia e Fisiologia do Exercício Equino (LAFEQ) Jaboticabal, São Paulo, Brazil., Garcia de Carvalho JR; Departamento de Morfologia e Fisiologia Animal, Faculdades de Ciências Agrárias e Veterinárias, UNESP Univ Estadual Paulista, Campus de Jaboticabal, Laboratório de Farmacologia e Fisiologia do Exercício Equino (LAFEQ) Jaboticabal, São Paulo, Brazil., Mendes de Almeida ML; Departamento de Tecnologia, Faculdades de Ciências Agrárias e Veterinárias, UNESP Univ Estadual Paulista, Campus de Jaboticabal, Jaboticabal, São Paulo, Brazil., Macedo Lemos EG; Departamento de Tecnologia, Faculdades de Ciências Agrárias e Veterinárias, UNESP Univ Estadual Paulista, Campus de Jaboticabal, Jaboticabal, São Paulo, Brazil., Brioschi Soares OA; Brazilain Army, Rio de Janeiro, RJ, Brazil., Ribeiro G; Faculdades Metropolitanas Unidas, HOVET, São Paulo, Brazil., de Queiroz-Neto A; Departamento de Morfologia e Fisiologia Animal, Faculdades de Ciências Agrárias e Veterinárias, UNESP Univ Estadual Paulista, Campus de Jaboticabal, Laboratório de Farmacologia e Fisiologia do Exercício Equino (LAFEQ) Jaboticabal, São Paulo, Brazil., de Camargo Ferraz G; Departamento de Morfologia e Fisiologia Animal, Faculdades de Ciências Agrárias e Veterinárias, UNESP Univ Estadual Paulista, Campus de Jaboticabal, Laboratório de Farmacologia e Fisiologia do Exercício Equino (LAFEQ) Jaboticabal, São Paulo, Brazil. Electronic address: guilherme.c.ferraz@unesp.br.
Jazyk: angličtina
Zdroj: Journal of equine veterinary science [J Equine Vet Sci] 2019 Oct; Vol. 81, pp. 102791. Date of Electronic Publication: 2019 Aug 26.
DOI: 10.1016/j.jevs.2019.102791
Abstrakt: We compare the expression levels of the lactate transporter complex consisting of the lactate transporter, monocarboxylate transporter 1 (MCT1), and its ancillary protein, cluster of differentiation 147 (CD147), in the membranes of red blood cells (RBCs) from two breeds of jumping horses and associate the expression levels of these proteins with their jumping ability. The expression levels of MCT1 and CD147 proteins on the membranes of RBCs collected from 30 show jumping horses of two different breeds were quantified: the Brazilian Sport Horses (n = 17) and the European Warmbloods (n = 13). The levels of MCT1 and CD147 in the RBC membranes were measured by Western blot using horse-specific antibodies. Statistical analyses included unpaired Student t-test and Chi-squared test. According to the expression levels of MCT1 and CD147 proteins, 88% of the Brazilian Sport Horses were categorized as high lactate transporters and the remaining 12% as low lactate transporters. The opposite was found for the European Warmbloods, where most animals (77%) were classified as low lactate transporters and the remaining animals (23%) were classified as high lactate transporters. Brazilian Sport Horses express statistically significantly higher levels of CD147 and MCT1 than European Warmbloods. The classification of horses considering the expression of proteins involved in the ability to transport lactate through the complex MCT1-CD147 seems to be breed dependent, with horses that are able to jump higher obstacles showing lower expression of the MCT1-CD147 complex in their RBCs.
(Copyright © 2019. Published by Elsevier Inc.)
Databáze: MEDLINE
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