| Autor: |
Ferns RB; Department of Medical Microbiology, Middlesex Hospital, University College Medical School, London, U.K., Tedder RS, Donoghue JL |
| Jazyk: |
angličtina |
| Zdroj: |
Journal of virological methods [J Virol Methods] 1988 Jun; Vol. 20 (2), pp. 143-53. |
| DOI: |
10.1016/0166-0934(88)90148-6 |
| Abstrakt: |
An anti-HIV 1 competitive ELISA was developed using a monoclonal anti-HIV 1 gag to capture viral antigen to the solid phase. This format of assay was compared with a competitive ELISA where a polyclonal human anti-HIV 1 was used, to capture the viral antigen. Several benefits were observed using the monoclonal-antibody form of the assay. Firstly, less viral antigen was needed on solid phase to give an equivalent test in terms of positive and negative optical density 450 nm values. Secondly, a slight increase in sensitivity was also gained without any loss of specificity and finally, as a result of the murine nature of the antibody on the solid phase, no cross-reaction with the labelled human antibody in the test conjugate was observed. Such cross-linking has been observed with the polyclonal form of the assay and can lead to false-negative reactions. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
|
