Bacterial community structure and response to nitrogen amendments in Lake Shenandoah (VA, USA).

Autor: Reynoso G; James Madison University, Harrisonburg, VA 22807, USA E-mail: steffemm@jmu.edu; Current address: Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA., Smith MR; James Madison University, Harrisonburg, VA 22807, USA E-mail: steffemm@jmu.edu; Current address: Texas A&M University, College Station, TX 77843, USA., Holmes CP 2nd; James Madison University, Harrisonburg, VA 22807, USA E-mail: steffemm@jmu.edu; Current address: Texas A&M University, College Station, TX 77843, USA., Keelan CR; James Madison University, Harrisonburg, VA 22807, USA E-mail: steffemm@jmu.edu., McGrath SE; James Madison University, Harrisonburg, VA 22807, USA E-mail: steffemm@jmu.edu., Alvarez GH; James Madison University, Harrisonburg, VA 22807, USA E-mail: steffemm@jmu.edu., Coceano MA; James Madison University, Harrisonburg, VA 22807, USA E-mail: steffemm@jmu.edu; Current address: University of Wyoming, Laramie, WY 82071, USA., Eldridge KA; James Madison University, Harrisonburg, VA 22807, USA E-mail: steffemm@jmu.edu., Fried HI; James Madison University, Harrisonburg, VA 22807, USA E-mail: steffemm@jmu.edu., Gilbert NE; James Madison University, Harrisonburg, VA 22807, USA E-mail: steffemm@jmu.edu; Current address: University of Tennessee, Knoxville, TN 37996, USA., Harris MT; James Madison University, Harrisonburg, VA 22807, USA E-mail: steffemm@jmu.edu., Kohler LR; James Madison University, Harrisonburg, VA 22807, USA E-mail: steffemm@jmu.edu; Current address: University of Kentucky, Lexington, KY 40508, USA., Modolo CM; James Madison University, Harrisonburg, VA 22807, USA E-mail: steffemm@jmu.edu., Murray EA; James Madison University, Harrisonburg, VA 22807, USA E-mail: steffemm@jmu.edu., Polisetti SM; James Madison University, Harrisonburg, VA 22807, USA E-mail: steffemm@jmu.edu., Sales DJ; James Madison University, Harrisonburg, VA 22807, USA E-mail: steffemm@jmu.edu., Walsh ES; James Madison University, Harrisonburg, VA 22807, USA E-mail: steffemm@jmu.edu., Steffen MM; James Madison University, Harrisonburg, VA 22807, USA E-mail: steffemm@jmu.edu.
Jazyk: angličtina
Zdroj: Water science and technology : a journal of the International Association on Water Pollution Research [Water Sci Technol] 2019 Aug; Vol. 80 (4), pp. 675-684.
DOI: 10.2166/wst.2019.311
Abstrakt: Microbial processes are critical to the function of freshwater ecosystems, yet we still do not fully understand the factors that shape freshwater microbial communities. Furthermore, freshwater ecosystems are particularly susceptible to effects of environmental change, including influx of exogenous nutrients such as nitrogen and phosphorus. To evaluate the impact of nitrogen loading on the microbial community structure of shallow freshwater lakes, water samples collected from Lake Shenandoah (Virginia, USA) were incubated with two concentrations of either ammonium, nitrate, or urea as a nitrogen source. The potential impact of these nitrogen compounds on the bacterial community structure was assessed via 16S rRNA amplicon sequencing. At the phylum level, the dominant taxa in Lake Shenandoah were comprised of Actinobacteria and Proteobacteria, which were not affected by exposure to the various nitrogen treatments. Overall, there was not a significant shift in the diversity of the bacterial community of Lake Shenandoah with the addition of nitrogen sources, indicating this shallow system may be constrained by other environmental factors.
Databáze: MEDLINE
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