Determination of rosamultin in rat plasma by LC-MS/MS and its application to a pharmacokinetic study.

Autor: Liu Q; Jiaozhou Central Hospital of Qingdao, Qingdao, China., Wang Y; Jiaozhou People's Hospital, Qingdao, China.
Jazyk: angličtina
Zdroj: Biomedical chromatography : BMC [Biomed Chromatogr] 2020 Feb; Vol. 34 (2), pp. e4728. Date of Electronic Publication: 2019 Dec 29.
DOI: 10.1002/bmc.4728
Abstrakt: A specific and reliable LC-MS/MS method for the determination of rosamultin in rat plasma was validated. Plasma samples were prepared with protein precipitation method, and chromatographic separation was performed on a Thermo C 18 analytical column (4.6 mm × 50 mm, 3.0 μm). The mass spectrometry (MS) analysis was conducted in positive SRM mode for the transitions of m/z 673.2 → 511.1 for rosamultin and m/z 601.1 → 330.9 for IS. The method validation was conducted over the calibration range of 1.0-500 ng/mL with the precision ≤11.03% and accuracy within ±14.64%. The assay was applied to the pharmacokinetic study after oral administration of rosamultin at a dose of 20 mg/kg in rats.
(© 2019 John Wiley & Sons, Ltd.)
Databáze: MEDLINE
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